DOI: 10.1016/j.watres.2018.09.057
Scopus记录号: 2-s2.0-85054328886
论文题名: UV inactivation of human infectious viruses at two full-scale wastewater treatment plants in Canada
作者: Qiu Y. ; Li Q. ; Lee B.E. ; Ruecker N.J. ; Neumann N.F. ; Ashbolt N.J. ; Pang X.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 147 起始页码: 73
结束页码: 81
语种: 英语
英文关键词: Cell culture
; Enteric viruses
; qPCR
; Reovirus
; UV disinfection
; Wastewater
英文摘要: Ultraviolet (UV) disinfection is widely used to inactivate microorganisms prior to release of treated municipal wastewater. However, limited data are available for in situ inactivation of infectious enteric viruses by UV treatment at full-scale. In this study, a total of 51 pre-UV and 50 post-UV samples were collected over a two-year period from two wastewater treatment plants (WWTPs) and analyzed for noroviruses, rotavirus, reovirus, sapovirus, astrovirus, enteroviruses, adenoviruses and JC virus. Both pre-UV and post-UV samples had relatively high concentrations of these viruses determined by qPCR. Infectious viruses were also observed in 98% of pre-UV samples and 76% of post-UV samples by cell culture, using either cytopathic effect (CPE) or integrated cell culture with qPCR (ICC-qPCR). Reovirus was the most common virus detected by ICC-qPCR, present in 92% of pre-UV and 48% of post-UV samples. Infectious enterovirus and adenovirus were detected by ICC-qPCR in 33% and 31% of pre-UV samples, 14% and 20% of post-UV samples, respectively. Mean log10 reduction estimates for infectious reovirus was 1.2 and 1.8 log for the two WWTPs as assessed by ICC-qPCR, which was similar to the reduction of total infectious viruses (1.5 and 1.7 log) as assessed by CPE in cells culture. Overall, quantification of infectious reovirus appears to provide a useful index of enteric virus inactivation during wastewater treatment at full-scale. To our knowledge, this is the first comprehensive study to assess UV inactivation of human enteric viruses at full-scale in WWTPs using both molecular and cell culture techniques, providing important information for quantitative microbial risk assessment of UV inactivation of human viruses in municipal wastewater. © 2018 Elsevier Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112354
Appears in Collections: 气候减缓与适应
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作者单位: Department of Laboratory Medicine and Pathology, University of Alberta, 116st & 85 Ave, Edmonton, AB T6G. 2R3, Canada; School of Public Health, University of Alberta, 11405-87Ave, Edmonton, AB T6G 1C9, Canada; Department of Pediatrics, University of Alberta, 11405-87Ave, Edmonton, AB T6G 1C9, Canada; City of Calgary, Water Resources, 625, 25 Avenue SE, Calgary, AB T2G 4K8, Canada; Provincial Laboratory for Public Health, 8440-112st, Edmonton, AB T6G 2J2, Canada
Recommended Citation:
Qiu Y.,Li Q.,Lee B.E.,et al. UV inactivation of human infectious viruses at two full-scale wastewater treatment plants in Canada[J]. Water Research,2018-01-01,147