DOI: 10.1016/j.watres.2018.02.057
Scopus记录号: 2-s2.0-85042496983
论文题名: Ciprofloxacin degradation in anaerobic sulfate-reducing bacteria (SRB) sludge system: Mechanism and pathways
作者: Jia Y. ; Khanal S.K. ; Shu H. ; Zhang H. ; Chen G.-H. ; Lu H.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 136 起始页码: 64
结束页码: 74
语种: 英语
英文关键词: Antibiotic resistance genes (ARGs)
; Antibiotics
; Biodegradation pathways
; Ciprofloxacin
; Sulfate-reducing bacteria (SRB)
Scopus关键词: Antibiotics
; Bacteria
; Biological water treatment
; Degradation
; Enzyme activity
; Genes
; Hydroxylation
; Sulfur compounds
; Wastewater treatment
; Antibiotic resistance genes
; Biodegradation pathways
; Ciprofloxacin
; Cytochrome p450 enzymes
; Extracellular environments
; Fluoroquinolone antibiotics
; Hydroxylation reactions
; Sulfate reducing bacteria
; Biodegradation
; bacterial DNA
; ciprofloxacin
; cytochrome P450
; quinoline derived antiinfective agent
; antiinfective agent
; ciprofloxacin
; sulfate
; antibiotic resistance
; antibiotics
; bacterium
; biodegradation
; concentration (composition)
; DNA
; gene
; sludge
; sulfur
; wastewater
; anaerobic bacterium
; antibiotic resistance
; Article
; bacterial cell
; bacterial gene
; batch process
; bioinformatics
; concentration (parameters)
; controlled study
; Desulfobacter
; DNA extraction
; gene dosage
; high throughput sequencing
; hydroxylation
; long term exposure
; microbial community
; microbial degradation
; nonhuman
; priority journal
; sludge
; sulfate reducing bacterium
; waste water management
; anaerobic growth
; analysis
; bioremediation
; classification
; Deltaproteobacteria
; genetics
; metabolism
; microbiology
; sewage
; waste water
; Desulfobacter
; Anaerobiosis
; Anti-Bacterial Agents
; Biodegradation, Environmental
; Ciprofloxacin
; Deltaproteobacteria
; Sewage
; Sulfates
; Waste Water
英文摘要: Ciprofloxacin (CIP), a fluoroquinolone antibiotic, removal was examined for the first time, in an anaerobic sulfate-reducing bacteria (SRB) sludge system. About 28.0% of CIP was biodegraded by SRB sludge when the influent CIP concentration was 5000 μg/L. Some SRB genera with high tolerance to CIP (i.e. Desulfobacter), were enriched at CIP concentration of 5000 μg/L. The changes in antibiotic resistance genes (ARGs) of SRB sludge coupled with CIP biodegradation intermediates were used to understand the mechanism of CIP biodegradation for the first time. The percentage of efflux pump genes associated with ARGs increased, while the percentage of fluoroquinolone resistance genes that inhibit the DNA copy of bacteria decreased during prolonged exposure to CIP. It implies that some intracellular CIP was extruded into extracellular environment of microbial cells via efflux pump genes to reduce fluoroquinolone resistance genes accumulation caused by exposure to CIP. Additionally, the degradation products and the possible pathways of CIP biodegradation were also examined using the new method developed in this study. The results suggest that CIP was biodegraded intracellularly via desethylation reaction in piperazinyl ring and hydroxylation reaction catalyzed by cytochrome P450 enzymes. This study provides an insight into the mechanism and pathways of CIP biodegradation by SRB sludge, and opens-up a new opportunity for the treatment of CIP-containing wastewater using sulfur-mediated biological process. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112862
Appears in Collections: 气候减缓与适应
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作者单位: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510275, China; Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, United States; State Key Laboratory of Biocontrol and Guangdong School of Life Sciences, Sun Yat-sen University, Guangzhou, China; Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
Recommended Citation:
Jia Y.,Khanal S.K.,Shu H.,et al. Ciprofloxacin degradation in anaerobic sulfate-reducing bacteria (SRB) sludge system: Mechanism and pathways[J]. Water Research,2018-01-01,136