DOI: 10.1016/j.watres.2017.12.057
Scopus记录号: 2-s2.0-85040066190
论文题名: Use of sperm DNA integrity as a marker for exposure to contamination in Palaemon serratus (Pennant 1777): Intrinsic variability, baseline level and in situ deployment
作者: Erraud A. ; Bonnard M. ; Chaumot A. ; Geffard O. ; Duflot A. ; Forget-Leray J. ; Le Foll F. ; Geffard A. ; Xuereb B.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 132 起始页码: 124
结束页码: 134
语种: 英语
英文关键词: Biomonitoring
; Comet assay
; Crustacean
; Environmental genotoxicity
; Reference
; Threshold value
Scopus关键词: Biomarkers
; Contamination
; Marine pollution
; Biomonitoring
; Comet assays
; Crustacean
; Environmental genotoxicity
; Reference
; Threshold-value
; DNA
; DNA
; biological marker
; baseline survey
; bioassay
; biomarker
; biomonitoring
; crustacean
; DNA
; genotoxicity
; in situ measurement
; optimization
; pollution effect
; sperm
; standard (reference)
; temporal variation
; threshold
; animal cell
; Article
; bay
; body size
; body weight
; controlled study
; DNA damage
; ecotoxicity
; environmental monitoring
; estuary
; genotoxicity
; male
; molting
; nonhuman
; Palaemon
; Palaemon serratus
; priority journal
; sperm quality
; water contamination
; water temperature
; animal
; comet assay
; DNA damage
; genetics
; metabolism
; Palaemonidae
; spermatozoon
; temperature
; Bay of Seine
; France
; Crustacea
; Decapoda (Crustacea)
; Palaemon serratus
; Animals
; Biomarkers
; Comet Assay
; DNA Damage
; Environmental Monitoring
; Estuaries
; Male
; Palaemonidae
; Spermatozoa
; Temperature
英文摘要: In a previous study, the Comet assay was optimized for Palaemon serratus prawns in order to propose a biomarker for sperm quality in this species. However, better knowledge of its basal level and its natural variability, related to intrinsic biotic and environmental abiotic factors, is required before any relevant use of this biomarker in the field. To fulfill this goal, the present study proceeded in three steps: (i) the temporal variability of DNA integrity was followed monthly in a reference population over a 2-year period, (ii) the correlation between the main intrinsic biotic (i.e. size, weight and molting stage) and abiotic factors (i.e. water temperature) were recorded in the field, and the basal DNA integrity was assessed in order to scrutinize any confounding influence of factors unrelated to toxic response, (iii) the baseline level was used to discriminate biomarker response among different stations displaying contrasting contamination levels. The results of the two-year monitoring in the reference population revealed no correlation between the levels of spermatozoa DNA damage and temperature, body size, weight or molting stage. Only a slight variability between monthly samplings was detected. On the basis of these field-collected data, we defined a reference distribution (i.e. 52.6 ± 5.6 A.U) with a threshold value (i.e. 61.7 A.U). Finally, this threshold value proved its relevance to discriminate among stations with contrasting pollution levels around the Seine Bay. Indeed, the results suggest significant DNA damage in populations nearest the Seine estuary, a major source of contaminants in the Bay, and a lower effect in populations further away from the estuary. The overall conclusion was that the Comet assay on P. serratus spermatozoa could be a useful tool for the monitoring of the toxicological print within sperm and main globally the contamination exposure of crustaceans in marine waters. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112962
Appears in Collections: 气候减缓与适应
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作者单位: Normandie Univ, UNIHAVRE, UMR-I 02 SEBIO, FR CNRS 3730 SCALE, Le Havre, 76600, France; Université Reims Champagne Ardenne, UMR-I 02 SEBIO, Reims, 51100, France; IRSTEA, UR MALY Laboratoire d’écotoxicologie, centre de Lyon-Villeurbanne, Villeurbanne, F-69616, France
Recommended Citation:
Erraud A.,Bonnard M.,Chaumot A.,et al. Use of sperm DNA integrity as a marker for exposure to contamination in Palaemon serratus (Pennant 1777): Intrinsic variability, baseline level and in situ deployment[J]. Water Research,2018-01-01,132