DOI: 10.1016/j.watres.2018.10.088
Scopus记录号: 2-s2.0-85057245978
论文题名: Comparative decay of sewage-associated marker genes in beach water and sediment in a subtropical region
作者: Ahmed W. ; Zhang Q. ; Kozak S. ; Beale D. ; Gyawali P. ; Sadowsky M.J. ; Simpson S.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 511
结束页码: 521
语种: 英语
英文关键词: Decay
; Health risks
; Marker genes
; Mesocosms
; Microbial source tracking
; Sewage contamination
Scopus关键词: Genes
; Health risks
; Polymerase chain reaction
; Risk assessment
; RNA
; Sediments
; Sewage
; Surface waters
; Tropics
; Water quality
; Decay
; Marker genes
; Mesocosms
; Microbial source tracking
; Sewage contamination
; Decay (organic)
; morphine sulfate
; RNA 16S
; RNA 23S
; sea water
; surface water
; biomarker
; coastal water
; coliform bacterium
; comparative study
; freshwater
; gene expression
; health risk
; mesocosm
; polymerase chain reaction
; resuspension
; sediment
; sewage treatment
; subtropical region
; tracking
; virus
; water column
; water quality
; Adenoviridae
; Article
; Bacteroides
; Enterococcus
; Escherichia coli
; marker gene
; mesocosm
; nonhuman
; Polyomavirus
; priority journal
; risk assessment
; seashore
; sediment
; sewage
; water quality
; water sampling
; Adenoviridae
; Bacteria (microorganisms)
; Bacteroides
; Enterococcus
; Polyomavirus
英文摘要: There is a growing move towards using the quantitative polymerase chain (qPCR)-based sewage-associated marker genes to assess surface water quality. However, a lack of understanding about the persistence of many sewage-associated markers creates uncertainty for those tasked with investigating microbial water quality. In this study, we investigated the decay of two qPCR FIB [E. coli (EC), and Enterococcus spp. (ENT) 23S rRNA genes] and four sewage-associated microbial source tracking (MST) marker genes [human Bacteroides HF183 16S rRNA, adenovirus (HAdV), and polyomavirus (HPyV), and crAssphage, a recently described bacteriophage in feces], in outdoor mesocosms containing fresh and marine waters and their corresponding sediments. Decay rates of EC 23S rRNA, ENT 23S rRNA, and HF183 16S rRNA were significantly (p < 0.05) faster than the HAdV, HPyV and crAssphage markers in water samples from all mesocosms. In general, decay rates of bacterial targets were similar in the water columns of the studied mesocosms. Similarly, decay rates of viral targets were also alike in mesocosm water columns in relation to each other. The decay rates of FIB and sewage-associated markers were significantly faster in water samples compared to sediments in all three mesocosms. In the event of resuspension, FIB and marker genes from sediments can potentially recontaminate overlying waters. Thus, care should be taken when interpreting the occurrence of FIB and sewage-associated MST markers in water, which may have originated from sediments. The differential decay of these targets may also influence health outcomes and need to be considered in risk assessment models. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122129
Appears in Collections: 气候变化事实与影响
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作者单位: CSIRO Land and Water, Ecosciences Precinct, 41 Boggo RoadQld 4102, Australia; BioTechnology Institute, Departments of Soil, Water & Climate, and Plant & Microbial Biology, University of Minnesota, St. Paul, MN 55108, United States; School of Medicine, Griffith University, Gold Coast, Australia; Institute of Environmental Science and Research Ltd., Kenepuru Science Center, Porirura, 5240, New Zealand; CSIRO Land and Water, Lucas HeightsNSW 2234, Australia
Recommended Citation:
Ahmed W.,Zhang Q.,Kozak S.,et al. Comparative decay of sewage-associated marker genes in beach water and sediment in a subtropical region[J]. Water Research,2019-01-01