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Eco-efficiency and building optimization potential of prefabricated structures to be used in new buildings are studied, focusing on the analysis of a novel dry precast beam-column connection under different durability and re-using scenarios. The analyses include structural response (via numerical models), optimization potential (material take off, building schedule and economic cost) and environmental impact (via Life Cycle Assessment, LCA). The connection is applied to a case study which is representative of common buildings: a seven-story concrete structure with frames and deck slabs. The structural response of the connection accomplishes the Eurocode safety prescriptions. Man-hours and task duration decrease around 80%, and the global schedule undergoes 60% diminution. Focusing on economic issues, when the prefabricated structural elements are reused the accumulated economic cost significantly decreases. LCA shows that durability, in terms of service life, is directly related to the environmental impact. However, other design options, such us re-using, have less repercussion in the impact categories (i.e. Global Warming Potential and Embodied Energy), and in the global cost. Results from this research could contribute to the implementation of prefabricated elements in the building stock promoting eco-efficiency.