DOI: 10.1016/j.meegid.2020.104206
论文题名: RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus
作者: Zheng X. ; Zhu Y. ; Zhao Z. ; Yan L. ; Xu T. ; Wang X. ; He H. ; Xia X. ; Zheng W. ; Xue X.
刊名: Infection, Genetics and Evolution
ISSN: 15671348
出版年: 2020
卷: 80 语种: 英语
英文关键词: Antiviral responses
; Canine distemper virus
; Differentially expression genes
; Macrophages
; Transcriptomic
Scopus关键词: chemokine
; cytokine
; cytokine receptor
; immunoglobulin enhancer binding protein
; nucleotide binding oligomerization domain like receptor
; pathogen associated molecular pattern
; pattern recognition receptor
; receptor
; RIG I like receptor
; toll like receptor
; tumor necrosis factor
; unclassified drug
; animal cell
; Article
; Canine distemper virus
; chemokine gene
; controlled study
; DH82 cell line
; differentially expressed gene
; dog breed
; down regulation
; gene
; gene control
; gene expression
; gene function
; gene ontology
; genetic association
; innate immunity
; interferon stimulated gene
; macrophage
; Morbillivirus infection
; nonhuman
; priority journal
; pro inflammatory factor genes
; protein protein interaction
; real time polymerase chain reaction
; RNA sequencing
; signal transduction
; transcriptomics
; upregulation
; virus cell interaction
英文摘要: Virulent morbillivirus infections, including Meals Virus (MeV) and Canine Distemper Virus (CDV), caused severe immune suppression and leukopenia, while attenuated vaccine strains developed protective host immune responses. However, the detailed molecular foundations of host antiviral responses were poorly characterized. In order to better understand the interactions between attenuated vaccine and host antiviral responses, the global gene expression changes in CDV-11-infected DH82 cells, a macrophage-derived cell line from canine, were investigated by transcriptomic analysis, and portions of results were confirmed with quantitative RT-PCR. The results exhibited that 372 genes significantly up-regulated (p < .01) and 119 genes were significantly down-regulated (p < .01) in CDV-infected macrophages DH82 at 48 h p.i. The enriched functions of the significantly up-regulated (p < .01) genes were closely associated with interferon stimulated genes (ISGs), chemokine genes and pro-inflammatory factor genes. Gene ontology and pathway analysis of differentially expressed genes (DEGs) revealed that the most significantly involved pathways in CDV-infected DH82 cells were NF-κB and TNF signaling pathway, cytokine-cytokine receptor interaction, and pathogen associated molecular patterns (PAMPs), such as Toll-like, RIG-I-like and NOD-like receptor signalings. Thus, the findings indicated that pattern recognition receptors (PRRs) possibly mediated host innate and protective antiviral immune responses in CDV-11 infected DH82 cells. © 2020 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158162
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: Department of Virology, School of Public Health, Shandong University, Jinan, 250012, China; Division of Infectious Diseases of Special Animal, Institute of Special Animal and Plant Sciences, The Chinese Academy of Agricultural Sciences, Changchun, 130122, China; Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China; College of Life Sciences, Shandong University, Qingdao, 266237, China; College of Life Sciences, Shandong Normal University, Jinan, 250014, China; Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, 130122, China
Recommended Citation:
Zheng X.,Zhu Y.,Zhao Z.,et al. RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus[J]. Infection, Genetics and Evolution,2020-01-01,80