DOI: 10.1016/j.envpol.2019.113647
论文题名: A global metabolomic insight into the oxidative stress and membrane damage of copper oxide nanoparticles and microparticles on microalga Chlorella vulgaris
作者: Wang L. ; Huang X. ; Sun W. ; Too H.Z. ; Laserna A.K.C. ; Li S.F.Y.
刊名: Environmental Pollution
ISSN: 2697491
出版年: 2020
卷: 258 语种: 英语
英文关键词: Glutathione metabolism
; High-resolution mass spectrometry
; Membrane lipid remodeling
; Metabolic pathway
; Nanotoxicity
; Osmoregulants
Scopus关键词: Algae
; Aquatic organisms
; Fatty acids
; Heavy ions
; Lipids
; Mass spectrometry
; Membranes
; Metabolism
; Metabolites
; Nanoparticles
; Oxidative stress
; Peptides
; Toxicity
; Glutathione metabolism
; High resolution mass spectrometry
; Membrane lipids
; Metabolic pathways
; Nanotoxicity
; Osmoregulants
; Copper oxides
; chemical compound
; chlorophyll
; copper ion
; copper oxide microparticle
; copper oxide nanoparticle
; diacylglycerol
; digalactosylmonoacylglycerol
; fatty acid
; galactosyldiacylglycerol
; glycerol
; lysophospholipid
; membrane lipid
; phosphatidic acid
; phosphatidylcholine
; phosphatidylethanolamine
; phosphatidylglycerol
; reactive oxygen metabolite
; copper compound
; green alga
; lipid
; mass spectrometry
; membrane
; metabolism
; microalga
; nanoparticle
; osmoregulation
; oxidative stress
; toxicity
; algal growth
; bioaccumulation
; Chlorella vulgaris
; controlled study
; exposure
; glutathione metabolism
; growth rate
; membrane damage
; metabolite
; metabolomics
; nonhuman
; oxidative stress
; particle size
; Chlorella vulgaris
英文摘要: Cu ions, CuO NPs and MPs induced perturbations of similar and various metabolic pathways related with oxidative stress and membrane damage in C. vulgaris. © 2019 Elsevier LtdTo compare aquatic organisms’ responses to the toxicity of copper oxide (CuO) nanoparticles (NPs) with those of CuO microparticles (MPs) and copper (Cu) ions, a global metabolomics approach was employed to investigate the changes of both polar and nonpolar metabolites in microalga Chlorella vulgaris after 5-day exposure to CuO NPs and MPs (1 and 10 mg/L), as well as the corresponding dissolved Cu ions (0.08 and 0.8 mg/L). Unchanged growth, slight reactive oxygen species production, and significant membrane damage (at 10 mg/L CuO particles) in C. vulgaris were demonstrated. A total of 75 differentiated metabolites were identified. Most metabolic pathways perturbed after CuO NPs exposure were shared by those after CuO MPs and Cu ions exposure, including accumulation of chlorophyll intermediates (max. 2.4–5.2 fold), membrane lipids remodeling for membrane protection (decrease of phosphatidylethanolamines (min. 0.6 fold) and phosphatidylcholines (min. 0.2–0.7 fold), as well as increase of phosphatidic acids (max. 1.5–2.9 fold), phosphatidylglycerols (max. 2.2–2.3 fold), monogalactosyldiacylglycerols (max. 1.2–1.4 fold), digalactosylmonoacylglycerols (max. 1.9–3.8 fold), diacylglycerols (max. 1.4 fold), lysophospholipids (max. 1.8–3.0 fold), and fatty acids (max. 3.0–6.2 fold)), perturbation of glutathione metabolism induced by oxidative stress, and accumulation of osmoregulants (max. 1.3–2.6 fold) to counteract osmotic stress. The only difference between metabolic responses to particles and those to ions was the accumulation of fatty acids oxidation products: particles caused higher fold changes (particles/ions ratio 1.9–3.0) at 1 mg/L and lower fold changes (particles/ions ratio 0.4–0.7) at 10 mg/L compared with ions. Compared with microparticles, there was no nanoparticle-specific pathway perturbed. These results confirm the predominant role of dissolved Cu ions on the toxicity of CuO NPs and MPs, and also reveal particle-specific toxicity from a metabolomics perspective. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158905
Appears in Collections: 气候变化与战略
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作者单位: MOE Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing, 100871, China; Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore; College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Peking University, Beijing, 100871, China; NUS Environmental Research Institute (NERI), #02-01, T-Lab Building (TL), 5A Engineering Drive 1, Singapore, 117411, Singapore
Recommended Citation:
Wang L.,Huang X.,Sun W.,et al. A global metabolomic insight into the oxidative stress and membrane damage of copper oxide nanoparticles and microparticles on microalga Chlorella vulgaris[J]. Environmental Pollution,2020-01-01,258