DOI: 10.1016/j.envres.2019.109080
论文题名: Global liver proteomic analysis of Wistar rats chronically exposed to low-levels of bisphenol A and S
作者: Azevedo L.F. ; Hornos Carneiro M.F. ; Dechandt C.R.P. ; Cassoli J.S. ; Alberici L.C. ; Barbosa F. ; Jr.
刊名: Environmental Research
ISSN: 139351
出版年: 2020
卷: 182 语种: 英语
英文关键词: Bisphenol A and S
; Effects
; Liver
; Metabolic homeostasis
; Proteomic analysis
Scopus关键词: 4,4' isopropylidenediphenol
; alanine aminotransferase
; aspartate aminotransferase
; binding protein
; bisphenol S
; complement component C1q
; cyclin dependent kinase 9
; death associated protein 3
; death associated protein kinase
; dystrophin
; dystrophin related protein 2
; endocrine disruptor
; glucokinase
; glucose
; high density lipoprotein cholesterol
; hydroxymethylglutaryl coenzyme A synthase
; liver protein
; low density lipoprotein cholesterol
; manganese superoxide dismutase
; mevalonic acid
; mitochondrial protein
; pyridoxal kinase
; reactive oxygen metabolite
; serotransferrin
; SET binding protein 1
; transferrin
; triacylglycerol
; ubiquilin 1
; ubiquilin 4
; unclassified drug
; unindexed drug
; dye
; ecotoxicology
; homeostasis
; metabolism
; physiology
; pollution effect
; pollution exposure
; protein
; proteomics
; rodent
; alanine aminotransferase blood level
; animal cell
; animal experiment
; animal tissue
; Article
; aspartate aminotransferase blood level
; blood biochemistry
; body weight gain
; controlled study
; detoxification
; gene ontology
; glucose blood level
; high density lipoprotein cholesterol level
; hypercholesterolemia
; hyperglycemia
; hypertriglyceridemia
; lipid metabolism
; lipogenesis
; liver toxicity
; long term exposure
; low density lipoprotein cholesterol level
; male
; mitochondrial respiration
; nonhuman
; priority journal
; protein expression
; protein function
; protein protein interaction
; proteomics
; rat
; respiratory chain
; total cholesterol level
; triacylglycerol blood level
; upregulation
; Wistar rat
; Rattus norvegicus
英文摘要: Exposure to bisphenol A (BPA) and bisphenol S (BPS) has been associated with the development of metabolic disorders, such as obesity, dyslipidemias, and nonalcoholic fatty liver disease. Nonetheless, the associated mechanisms are still not fully understood. BPS is being used with no restrictions to replace BPA, which increases the concern regarding its safety and claims for further investigation on its potential mechanisms of toxicity. The present study aims to access liver molecular disturbances which could be associated with systemic metabolic disorders following exposure to BPA or BPS. Therefore, body weight gain and serum biochemical parameters were measured in male Wistar rats chronically exposed to 50 or 500 µg/kg/day of BPA or BPS, while an extensive evaluation of liver protein expression changes was conducted after exposure to 50 µg/kg/day of both compounds. Exposure to the lowest dose of BPA led to the development of hyperglycemia and hypercholesterolemia, while the BPS lowest dose led to the development of hypertriglyceridemia. Besides, exposure to 500 µg/kg/day of BPS significantly increased body weight gain and LDL-cholesterol levels. Hepatic proteins differentially expressed in BPA and BPS-exposed groups compared to the control group were mostly related to lipid metabolism and synthesis, with upregulation of glucokinase activity-related sequence 1 (1.8-fold in BPA and 2.4-fold in BPS), which is involved in glycerol triglycerides synthesis, and hydroxymethylglutaryl-CoA synthase cytoplasmic (2-fold in BPS), an enzyme involved in mevalonate biosynthesis. Essential mitochondrial proteins of the electron transport chain were upregulated after exposure to both contaminants. Also, BPA and BPS dysregulated expression of liver antioxidant enzymes, which are involved in cellular reactive oxygen species detoxification. Altogether, the results of the present study contribute to expand the scientific understanding of how BPA and BPS lead to the development of metabolic disorders and reinforce the risks associated with exposure to these contaminants. © 2019 Elsevier Inc. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158969
Appears in Collections: 气候变化与战略
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作者单位: Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, Brazil; Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, Brazil; Institute of Biological Sciences, Federal University of Pará, Belém, Brazil; Department of Pharmacy, Faculty of Chemistry and Pharmacy, Pontifical Catholic University of Chile, Santiago, 7820436, Chile
Recommended Citation:
Azevedo L.F.,Hornos Carneiro M.F.,Dechandt C.R.P.,et al. Global liver proteomic analysis of Wistar rats chronically exposed to low-levels of bisphenol A and S[J]. Environmental Research,2020-01-01,182