globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1808200116
论文题名:
Escape band in Escherichia coli chemotaxis in opposing attractant and nutrient gradients
作者: Zhang X.; Si G.; Dong Y.; Chen K.; Ouyang Q.; Luo C.; Tu Y.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:6
起始页码: 2253
结束页码: 2258
语种: 英语
英文关键词: Chemotaxis ; Growth ; Opposing gradients ; Traveling wave
Scopus关键词: aspartic acid ; chemoattractant ; methylaspartic acid ; receptor ; Tap receptor ; Tar receptor ; unclassified drug ; chemotactic factor ; Article ; bacterial growth ; bacterial phenomena and functions ; bacterium mutant ; broth dilution ; cell density ; chemotaxis ; controlled study ; critical value ; escape band ; Escherichia coli ; mathematical model ; microfluidics ; nonhuman ; nutrient ; priority journal ; quantitative analysis ; algorithm ; biological model ; chemotaxis ; Escherichia coli ; immunology ; metabolism ; microfluidic analysis ; physiology ; reproducibility ; Algorithms ; Chemotactic Factors ; Chemotaxis ; Escherichia coli ; Microfluidic Analytical Techniques ; Models, Biological ; Nutrients ; Reproducibility of Results
英文摘要: It is commonly believed that bacterial chemotaxis helps cells find food. However, not all attractants are nutrients, and not all nutrients are strong attractants. Here, by using microfluidic experiments, we studied Escherichia coli chemotaxis behavior in the presence of a strong chemoattractant (e.g., aspartate or methylaspartate) gradient and an opposing gradient of diluted tryptone broth (TB) growth medium. Our experiments showed that cells initially accumulate near the strong attractant source. However, after the peak cell density (h) reaches a critical value hc, the cells form a “escape band” (EB) that moves toward the chemotactically weaker but metabolically richer nutrient source. By using various mutant strains and varying experimental conditions, we showed that the competition between Tap and Tar receptors is the key molecular mechanism underlying the formation of the escape band. A mathematical model combining chemotaxis signaling and cell growth was developed to explain the experiments quantitatively. The model also predicted that the width w and the peak position xp of EB satisfy two scaling relations: w/l ∼ (h/hc)−1/ 2 and 1 − xp/l ∼ (h/hc)−1/ 2 , where l is the channel length. Both scaling relations were verified by experiments. Our study shows that the combination of nutrient consumption, population growth, and chemotaxis with multiple receptors allows cells to search for optimal growth condition in complex environments with conflicting sources. © 2019 National Academy of Sciences. All Rights Reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163597
Appears in Collections:气候变化与战略

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作者单位: Zhang, X., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Si, G., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Dong, Y., Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Chen, K., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China; Ouyang, Q., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Peking-Tsinghua Center for Life Science, Peking University, Beijing, 100871, China; Luo, C., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Tu, Y., Physical Sciences Department, IBM T. J. Watson Research Center, Yorktown Heights, NY 10598, United States

Recommended Citation:
Zhang X.,Si G.,Dong Y.,et al. Escape band in Escherichia coli chemotaxis in opposing attractant and nutrient gradients[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(6)
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