globalchange  > 气候变化与战略
DOI: 10.1016/j.scib.2021.01.013
论文题名:
SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production
作者: Luo L.; Li Z.; Zhao T.; Ju X.; Ma P.; Jin B.; Zhou Y.; He S.; Huang J.; Xu X.; Zou Y.; Li P.; Liang A.; Liu J.; Chi T.; Huang X.; Ding Q.; Jin Z.; Huang C.; Zhang Y.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2021
卷: 66, 期:12
起始页码: 1194
结束页码: 1204
语种: 英语
中文关键词: G3BP ; Liquid-liquid phase separation ; Nucleocapsid protein ; SARS-CoV-2 ; Stress granules ; Viral production
英文关键词: Diseases ; Granulation ; Liquids ; Proteins ; Antivirals ; Coronaviruses ; G3BP ; Liquid-liquid phase separation ; Nucleocapsid proteins ; Protein phasis ; Severe acute respiratory syndrome coronavirus ; Severe acute respiratory syndrome coronavirus 2 ; Stress granule ; Viral production ; Phase separation
英文摘要: A key to tackling the coronavirus disease 2019 (COVID-19) pandemic is to understand how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manages to outsmart host antiviral defense mechanisms. Stress granules (SGs), which are assembled during viral infection and function to sequester host and viral mRNAs and proteins, are part of the antiviral responses. Here, we show that the SARS-CoV-2 nucleocapsid (N) protein, an RNA binding protein essential for viral production, interacted with Ras-GTPase-activating protein SH3-domain-binding protein (G3BP) and disrupted SG assembly, both of which require intrinsically disordered region1 (IDR1) in N protein. The N protein partitioned into SGs through liquid-liquid phase separation with G3BP, and blocked the interaction of G3BP1 with other SG-related proteins. Moreover, the N protein domains important for phase separation with G3BP and SG disassembly were required for SARS-CoV-2 viral production. We propose that N protein-mediated SG disassembly is crucial for SARS-CoV-2 production. © 2021 Science China Press
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170350
Appears in Collections:气候变化与战略

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作者单位: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China; Center for Infectious Disease Research, School of Medicine, Tsinghua University, Beijing, 100084, China; Department of Hematology, Tongji Hospital of Tongji University, Shanghai, 200065, China; Beijing Advanced Innovation Center for Structural Biology, Tsinghua University, Beijing, 100084, China

Recommended Citation:
Luo L.,Li Z.,Zhao T.,et al. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production[J]. Science Bulletin,2021-01-01,66(12)
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