| DOI: | 10.2172/985244
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| 报告号: | LBNL-3640E-Poster
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| 报告题名: | Revisiting Modes of energy generation in sulfate reducing bacteria |
| 作者: | Joachimiak, Marcin; Chakraborty, Romy; Zhou, Aifen; Fortney, Julian; Geller, Jil; Wall, Judy; Zhou, Jizhong; Arkin, Adam; Hazen, Terry; Keasling, Jay; Chhabra, Swapnil
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| 出版年: | 2010
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| 发表日期: | 2010-05-17
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | Sulfate reducing bacteria
; mineralize organic matter
; Desulfovibrio vulgaris strain Hildenborough (DvH)
|
| 中文主题词: | 碳
; 有机物
; 硫磺
; 密度
; 金属
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| 主题词: | CARBON
; ORGANIC MATTER
; SULFUR
; DENSITY
; METALS
|
| 英文摘要: | Sulfate reducing bacteria (SRB) play an important role in global sulfur and carbon cycling through their ability to completely mineralize organic matter while respiring sulfate to hydrogen sulfide. They are ubiquitous in anaerobic environments and have the ability to reduce toxic metals like Cr(VI) and U(VI). While SRB have been studied for over three decades, bioenergetic modes of this group of microbes are poorly understood. Desulfovibrio vulgaris strain Hildenborough (DvH) has served as a model SRB over the last decade with the accumulation of transcriptomic, proteomic and metabolic data under a wide variety of stressors. To further investigate the three hypothesized modes of energy generation in this anaerobe we conducted a systematic study involving multiple electron donor and acceptor combinations for growth. DvH was grown at 37oC in a defined medium with (a) lactate + thiosulfate, (b) lactate + sulfite (c) lactate + sulfate, (d) pyruvate + sulfate, (e) H2 + acetate + sulfate, (f) formate + acetate + sulfate, g) formate + sulfate and (h) pyruvate fermentation. Cells were harvested at mid-log phase of growth for all conditions for transcriptomics, when the optical density at 600nm was in the range 0.42-0.5. Initial results indicate that cells grown on lactate do not appear to significantly differentiate their gene expression profiles when presented with different electron acceptors. These profiles however differ significantly from those observed during growth with other electron donors such as H2 and formate, as well as during fermentative growth. Together the gene expression changes in the presence of different electron donors provide insights into the ability of DvH to differentially reduce metals such as Cr(VI). Here we present revised modes of energy generation in DvH in light of this new transcriptomic evidence. |
| URL: | http://www.osti.gov/scitech/servlets/purl/985244
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/39812
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| File Name/ File Size |
Content Type |
Version |
Access |
License |
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| 985244.pdf（21032KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Joachimiak, Marcin,Chakraborty, Romy,Zhou, Aifen,et al. Revisiting Modes of energy generation in sulfate reducing bacteria. 2010-01-01.
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