DOI: 10.1016/j.watres.2018.09.046
Scopus记录号: 2-s2.0-85054064846
论文题名: Inhibitory effects of ammonia on syntrophic propionate oxidation in anaerobic digester sludge
作者: Zhang C. ; Yuan Q. ; Lu Y.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 146 起始页码: 275
结束页码: 287
语种: 英语
英文关键词: Ammonia stress
; Anaerobic digester
; Methanogenesis
; Stable isotope probe
; Syntrophic propionate oxidation
英文摘要: Syntrophic propionate oxidation (SPO) coupled with methanogenesis is often inhibited under high ammonium concentrations in anaerobic digesters. However, the inhibitory mechanism remains poorly understood. We conducted two independent laboratory experiments with a swine manure digester sludge. In experiment I, RNA-based stable isotope probing (SIP) was applied to determine the active players of both bacteria and methanogens involved in SPO under different ammonium concentrations (0, 3 and 7 g NH4 +–N L−1). In experiment II, the dynamics of the bacterial community under ammonia stress was monitored using the 16S rRNA pyrosequencing and quantitative PCR under similar conditions as in experiment I but without the addition of external propionate. An additional higher ammonium treatment (10 g NH4 +–N L−1) was applied in experiment II to maximize the ammonia stress. We identified that the Smithella bacteria and the Methanosaetaceae and Methanospirillaceae archaea were the most active players involved in SPO and methanogenesis. We revealed that Smithella, Methanosaetaceae and Methanospirillaceae were moderately and severely inhibited at 3 and 7–10 g NH4 +–N L−1, respectively. However, the fermentative bacteria appeared to be more tolerant to ammonia stress. The microbial responses were corroborated with the accumulation of VFAs and the repression of methanogenesis under high ammonium conditions. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112386
Appears in Collections: 气候减缓与适应
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作者单位: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China; College of Urban and Environmental Sciences, Peking University, Beijing, 100871, China; State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, China
Recommended Citation:
Zhang C.,Yuan Q.,Lu Y.. Inhibitory effects of ammonia on syntrophic propionate oxidation in anaerobic digester sludge[J]. Water Research,2018-01-01,146