DOI: 10.1016/j.watres.2018.08.030
Scopus记录号: 2-s2.0-85053057607
论文题名: Applying UV absorbance and fluorescence indices to estimate inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluent
作者: Wu J. ; Cheng S. ; Cai M.-H. ; Wu Y.-P. ; Li Y. ; Wu J.-C. ; Li A.-M. ; Li W.-T.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 145 起始页码: 354
结束页码: 364
语种: 英语
英文关键词: Bacterial inactivation
; Bromate
; Flow cytometry
; Humic-like fluorescence
; Ozonation
; UV absorbance
Scopus关键词: Byproducts
; Efficiency
; Effluents
; Escherichia coli
; Flow cytometry
; Fluorescence
; Ion chromatography
; Ozone
; Ozonization
; Antibiotic resistance genes
; Autochthonous bacteria
; Bacterial inactivation
; Bromate
; Escherichia coli (E. coli)
; Fluorescence staining
; Inactivation efficiency
; UV absorbance
; Ozone water treatment
; absorbance
; antibiotic resistance
; coliform bacterium
; effluent
; estimation method
; flow cytometry
; fluorescence
; humic substance
; ozonation
; ozone
; ultraviolet radiation
; wastewater treatment
; Bacteria (microorganisms)
; Escherichia coli
英文摘要: Ozone is an effective oxidant and disinfectant commonly used for elimination of micropollutants and inactivation of resistant microbes. However, undesirable oxidation/disinfection byproducts such as bromate might form during ozonation. In this study, the UV absorbance and fluorescence indices were applied as surrogate indicators for predicting the inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluents. The inactivation efficiencies of lab-cultured Escherichia coli (E. coli) and autochthonous bacteria were measured by plating (for E. coli only) and flow cytometry with fluorescence staining. During ozonation of E. coli spiked into wastewater effluents (∼106 cell/mL), the priority of inactivation efficiency determined by different cell viability methods were in the order of CFU > membrane damage > DNA damage. Approximately, 99% membrane damage and/or 90% DNA damage are conservatively supposed as an indicator for sufficient bacterial inactivation as well as degradation of antibiotic resistance genes. The related required O3 dosing thresholds for sufficient inactivation of E. coli and autochthonous bacteria refer to ∼0.6 O3/DOC (g/g), ∼50% decrease of UVA254, ∼60% decrease of UVA280, or ∼80% decrease of humic-like fluorescence. Within the range of 106–108 cell/mL, the bacterial concentration did not have significant effects on the required thresholds of the specific O3 doses or spectroscopic indicators required for bacterial inactivation. The addition of 50 mM tert-BuOH as ·OH scavenger increased the required specific ozone doses but decreased the losses of spectroscopic indicators necessary for sufficient bacterial inactivation, and also suggested that the membrane/DNA damages for bacterial inactivation were mainly attributed to the direct O3 attacks. The bromate concentration was determined using ion chromatography with MS/MS detection. The results showed that when O3 was dosed at the required thresholds for sufficient bacterial inactivation, bromate formation could usually be suppressed below 10 μg/L. The present work supports that it is possible to reach a balance between bacterial inactivation and bromate formation. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112435
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China; Key Laboratory of Surficial Geochemistry Ministry of Education, School of Earth Sciences and Engineering, Hydrosciences Department, Nanjing University, Nanjing, 210023, China
Recommended Citation:
Wu J.,Cheng S.,Cai M.-H.,et al. Applying UV absorbance and fluorescence indices to estimate inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluent[J]. Water Research,2018-01-01,145