DOI: 10.1016/j.watres.2018.08.042
Scopus记录号: 2-s2.0-85052968582
论文题名: Diversity of potential antibiotic-resistant bacterial pathogens and the effect of suspended particles on the spread of antibiotic resistance in urban recreational water
作者: Fang T. ; Wang H. ; Cui Q. ; Rogers M. ; Dong P.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 145 起始页码: 541
结束页码: 551
语种: 英语
英文关键词: Antibiotic resistance genes
; Antibiotic-resistant bacterial pathogens
; Spread of antibiotic resistance
; Suspended particles
; Urban recreational waters
Scopus关键词: Genes
; Health risks
; Pathogens
; Polymerase chain reaction
; Water management
; Antibiotic resistance
; Antibiotic resistance genes
; Antibiotic-resistant bacterial pathogens
; Recreational water
; Suspended particles
; Antibiotics
; antibiotic resistance
; bacterium
; community composition
; concentration (composition)
; environmental factor
; lake water
; pathogen
; suspended particulate matter
; urban area
; water
; watershed
; Beijing [China]
; China
; Acinetobacter junii
; Bacteria (microorganisms)
英文摘要: Evidence of the increasing incidence of antibiotic resistance in watersheds has attracted worldwide attention. Limited in formation is available on the occurrences of health-related antibiotic-resistant bacterial pathogens (ARBPs) in recreational waters. The effects of certain environmental factors (e.g., suspended particles) on the spread of resistance also has not been characterized to date. In this study, a combination of culture and molecular methods was employed to comprehensively investigate the patterns of microbial resistance to representative antibiotics in samples from three recreational lakes in Beijing. The antibiotic resistance index (ARI) based on the gradient concentration assay revealed that samples showed high resistance to penicillin-G, moderate resistance to ampicillin, vancomycin and erythromycin and low resistance to ceftriaxone, gentamycin, tetracycline and chloramphenicol. Antibiotic-resistant bacteria (ARB) were cultured and collected, and the diversity of potential ARBP species was further explored using next-generation sequencing (NGS). The results showed that most of the identified ARBPs were environmental opportunistic pathogens with emerging clinical concerns, e.g., the multidrug-resistant Acinetobacter junii. Furthermore, particle-attached (PA) fractions presented higher ARI values than free-floating (FL) fractions did, indicating that the PA fractions were more resistant to selected antibiotics. And the NGS results revealed that the PA fractions showed higher similarity in the screened ARB community compositions in comparison with the FL fractions, primarily due to a protective effect provided by the particles. Accordingly, ARBPs could persist for a longer time in protective particle matrices. However, quantification of antibiotic-resistant genes (ARGs) by qPCR showed no significant abundance differences between the two fractions. Overall, these findings suggest a potential health risk from the prevalence of ARBPs in recreational waters and provides a better understanding of the contribution of particles in the spread of antibiotic resistance in aquatic systems, with implications for the control of excessive suspended particles by water management. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112440
Appears in Collections: 气候减缓与适应
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作者单位: State Key Joint Laboratory on Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, 100084, China; Chinese Research Academy of Environmental Sciences, Beijing, 100012, China; Department of Civil and Environmental Engineering, National University of Singapore, Engineering Drive 3117576, Singapore
Recommended Citation:
Fang T.,Wang H.,Cui Q.,et al. Diversity of potential antibiotic-resistant bacterial pathogens and the effect of suspended particles on the spread of antibiotic resistance in urban recreational water[J]. Water Research,2018-01-01,145