DOI: 10.1016/j.watres.2018.07.036
Scopus记录号: 2-s2.0-85050161255
论文题名: Coupling magnetic-nanoparticle mediated isolation (MMI) and stable isotope probing (SIP) for identifying and isolating the active microbes involved in phenanthrene degradation in wastewater with higher resolution and accuracy
作者: Li J. ; Luo C. ; Zhang G. ; Zhang D.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 144 起始页码: 226
结束页码: 234
语种: 英语
英文关键词: Active microbes for phenanthrene degradation
; Magnetic-nanoparticle mediated isolation (MMI)
; MMI-SIP
; PAH-RHDα genes
; Stable isotope probing (SIP)
Scopus关键词: Anthracene
; DNA
; Genes
; Isotopes
; Metabolism
; Nanomagnetics
; Nanoparticles
; Physiology
; Wastewater treatment
; Contaminated wastewater
; Effective management
; Higher resolution
; Magnetic nano-particles
; Microbial communities
; Natural habitat
; Stable-isotope probing
; Wastewater treatment plants
; Bacteria
; magnetic nanoparticle
; phenanthrene
; stable isotope
; accuracy assessment
; bacterium
; biodegradation
; degradation
; enzyme
; enzyme activity
; metabolism
; microbial activity
; microbial community
; microbial ecology
; microorganism
; phenanthrene
; stable isotope
; wastewater
; wastewater treatment
; wastewater treatment plant
; accuracy
; Article
; degradation
; isolation
; isotope analysis
; isotope labeling
; magnetic field
; magnetic nanoparticle mediated isolation
; microorganism detection
; nonhuman
; priority journal
; stable isotope probing
; waste water
; Pigmentiphaga
; Pseudomonas
; Sphingobium
英文摘要: Stable isotope probing (SIP) is a cultivation-independent approach identifying the functional microbes in their natural habitats, possibly linking their identities to functions. DNA-SIP is well-established but suffers from the shift of 12C-DNA into the heavy DNA (13C-DNA) fraction, which significantly reduces the resolution and accuracy. In this study, we coupled magnetic-nanoparticle mediated isolation (MMI) and DNA-SIP, namely MMI-SIP, to identify the active microbes involved in phenanthrene degradation from PAH-contaminated wastewater. Microbes affiliated to Pseudomonas and Sphingobium were responsible for in situ phenanthrene metabolism from the SIP results, and Pigmentiphaga was only unraveled for phenanthrene degradation in the MMI and MMI-SIP microcosms. MMI-SIP also significantly increased the enrichment of the above microbes and genes encoding the alpha subunit of the PAH-ring hydroxylating dioxygenase (PAH-RHDα) in the heavy DNA fractions. Our findings suggest that MMI-SIP is a powerful tool, with higher resolution and accuracy, to distinguish the active microbes involved in phenanthrene metabolism in the wastewater, provide a more precise map of functional microbial communities, and offer suggestions for effective management for wastewater treatment plants. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112481
Appears in Collections: 气候减缓与适应
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作者单位: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China; University of Chinese Academy of Sciences, Beijing, 100039, China; College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China; School of Environment, Tsinghua University, Beijing, 100084, China
Recommended Citation:
Li J.,Luo C.,Zhang G.,et al. Coupling magnetic-nanoparticle mediated isolation (MMI) and stable isotope probing (SIP) for identifying and isolating the active microbes involved in phenanthrene degradation in wastewater with higher resolution and accuracy[J]. Water Research,2018-01-01,144