DOI: 10.1016/j.watres.2018.06.053
Scopus记录号: 2-s2.0-85051032781
论文题名: Interactions of functional bacteria and their contributions to the performance in integrated autotrophic and heterotrophic denitrification
作者: Zhang R.-C. ; Xu X.-J. ; Chen C. ; Xing D.-F. ; Shao B. ; Liu W.-Z. ; Wang A.-J. ; Lee D.-J. ; Ren N.-Q.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 143 起始页码: 355
结束页码: 366
语种: 英语
英文关键词: Heterotrophic nitrate-reducing bacteria (hNRB)
; Integrated autotrophic and heterotrophic denitrification
; Microbial interactions
; Sulfide-oxidizing, nitrate-reducing bacteria (soNRB)
; Sulfur transformation
Scopus关键词: Bioconversion
; Bioreactors
; Carbon dioxide
; Carbon inorganic compounds
; Denitrification
; Nitrates
; Organic carbon
; Sulfur dioxide
; Denitrifying sulfide removal
; Functional mechanisms
; Heterotrophic denitrification
; Heterotrophic denitrifiers
; Microbial community structures
; Microbial interactions
; Nitrate-reducing bacteria
; Sulfur transformation
; Bacteria
; bacterium
; bioreactor
; biotransformation
; community structure
; concentration (composition)
; denitrification
; functional group
; microbial activity
; microbial community
; microbial ecology
; organic carbon
; performance assessment
; sulfur
; wastewater
; wastewater treatment
; Azoarcus
; Pseudomonas
; Thauera
; Thiobacillus
英文摘要: Compared to autotrophic and heterotrophic denitrification process, the Integrated autotrophic and heterotrophic denitrification (IAHD) has wider foreground of applications in the condition where the organic carbon, nitrate and inorganic sulfur compounds usually co-exist in the actual wastewaters. As the most well-known IAHD process, the denitrifying sulfide removal (DSR) could simultaneously convert sulfide, nitrate and organic carbon into sulfur, dinitrogen gas and carbon dioxide, respectively. Thus, systematical metabolic functions and contributions of autotrophic and heterotrophic denitrifiers to the IAHD-DSR performance became an problem demanding to be promptly studied. In this work, three upflow anaerobic sludge bioreactors (UASBs) were individually started up in autotrophic (a-DSR), heterotrophic (h-DSR) and mixotrophic conditions (m-DSR). Then, the operating conditions of each bioreactor were switched to different trophic conditions with low and high sulfide concentrations in the influent (200 and 400 mg/L). The removal efficiencies of sulfide, nitrate and acetate all reached 100% in all three bioreactors throughout the operational stages. However, the sulfur transformation ratio ranged from 34.5% to 39.9% at the low sulfide concentration and from 76.8% to 86.7% at the high sulfide concentration in the mixotrophic conditions. Microbial community structure analyzed by the Illumina sequencing indicated that Thiobacillus, which are autotrophic sulfide-oxidizing, nitrate-reducing bacteria (a-soNRB), was the dominant genus (81.3%) in the a-DSR bioreactor. With respect to the mixotrophic conditions, at low sulfide concentration in the m-DSR bioreactor, Thiobacillus (a-soNRB) and Thauera, which are heterotrophic nitrate-reducing bacteria (hNRB), were the dominant genera, with percentages of 48.8% and 14.9%, respectively. When the sulfide concentration in the influent was doubled, the percentage of Thiobacillus decreased by approximately 9-fold (from 48.8% to 5.4%), and the total percentage of Azoarcus and Pseudomonas, which are heterotrophic sulfide-oxidizing, nitrate-reducing bacteria (h-soNRB), increased by approximately 6-fold (from 10.1% to 59.4%). Therefore, the following interactions between functional groups and their functional mechanisms in the DSR process were proposed: (1) a-soNRB (Thiobacillus) and hNRB (Thauera) worked together to maintain the performance under the low sulfide concentration; (2) h-soNRB (Azoarcus and Pseudomonas) took the place of a-soNRB and worked together with hNRB (Thauera and Allidiomarina) under the high sulfide concentration; and (3) a-soNRB (such as Thiobacillus) were possibly the key bacteria and may have contributed to the low sulfur transformation, and h-soNRB may be responsible for the high sulfur transformation in the DSR process. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112541
Appears in Collections: 气候减缓与适应
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作者单位: State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, Heilongjiang Province 150090, China; Department of Chemical Engineering, National Taiwan University, Taipei, 106, Taiwan; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China
Recommended Citation:
Zhang R.-C.,Xu X.-J.,Chen C.,et al. Interactions of functional bacteria and their contributions to the performance in integrated autotrophic and heterotrophic denitrification[J]. Water Research,2018-01-01,143