DOI: 10.1016/j.watres.2018.03.070
Scopus记录号: 2-s2.0-85053041450
论文题名: A novel environmental fate of graphene oxide: Biodegradation by a bacterium Labrys sp. WJW to support growth
作者: Qu Y. ; Wang J. ; Ma Q. ; Shen W. ; Pei X. ; You S. ; Yin Q. ; Li X.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 143 起始页码: 260
结束页码: 269
语种: 英语
英文关键词: Bacterium
; Biodegradation
; Carbon source
; Graphene oxide
; Labrys sp.
; Proteomic
Scopus关键词: Atomic force microscopy
; Bacteria
; Benzoic acid
; Gas chromatography
; Graphene
; Graphene oxide
; High resolution transmission electron microscopy
; Liquid chromatography
; Mass spectrometry
; Polymerase chain reaction
; Proteins
; Reaction intermediates
; Transmission electron microscopy
; X ray photoelectron spectroscopy
; Bioinformatics analysis
; Carbon source
; Diverse applications
; Gas chromatographs
; Labrys sp
; Physicochemical property
; Proteomic
; Sole carbon source
; Biodegradation
; bacterium
; biodegradation
; bioinformatics
; environmental fate
; genomics
; growth
; microbial activity
; organic compound
; physicochemical property
; protein
; proteomics
; Bacteria (microorganisms)
; Labrys
英文摘要: Graphene oxide (GO) is a new type of nanomaterial with unique physicochemical properties and diverse applications, whereas it poses potential risk to human and environment. By screening from natural soil exposed to GO in the laboratory, we successfully obtained a novel bacterium, Labrys sp. WJW, which was able to use GO as the sole carbon source for growth. Within 8 days, cell numbers increased 16.76 ± 3.21 folds using 100 mg/L GO as the carbon source by qPCR analysis. The bacterial biodegradation which resulted in formation of holes and functional group changes of GO was proved by Raman spectroscopy, atomic force microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy analyses. Aromatic intermediates with structures of benzoic acid and phenol were identified using gas chromatograph-mass spectrometry and liquid chromatography/time-of-flight/mass spectrometry. Combination of genomic and proteomic analyses were performed to explore the proteins associated with GO degradation. A total of 644 proteins were significantly shifted. Bioinformatics analysis indicated that part of the up-regulated proteins were related to oxidation, ring cleavage and intermediates transmembrane processes, and GO was supposed to be degraded to benzoate and further degraded for downstream processes. This study enriches our understanding and provides new insights into the environmental fate of GO. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112570
Appears in Collections: 气候减缓与适应
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作者单位: Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China; Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China
Recommended Citation:
Qu Y.,Wang J.,Ma Q.,et al. A novel environmental fate of graphene oxide: Biodegradation by a bacterium Labrys sp. WJW to support growth[J]. Water Research,2018-01-01,143