DOI: 10.1016/j.watres.2018.05.055
Scopus记录号: 2-s2.0-85049352776
论文题名: Induction of Escherichia coli into a VBNC state through chlorination/chloramination and differences in characteristics of the bacterium between states
作者: Chen S. ; Li X. ; Wang Y. ; Zeng J. ; Ye C. ; Li X. ; Guo L. ; Zhang S. ; Yu X.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 142 起始页码: 279
结束页码: 288
语种: 英语
英文关键词: Chloramination
; Chlorination
; Induction
; Resuscitation
; VBNC
Scopus关键词: Amination
; Chlorination
; Chlorine
; Condensation reactions
; Cytology
; Disinfection
; Escherichia coli
; Health risks
; Heavy water
; Metabolism
; Potable water
; Resuscitation
; Scanning electron microscopy
; Water distribution systems
; Water pollution control
; Chloramination
; Drinking water disinfection
; Induction
; Laser confocal microscopy
; Luria-Bertani broths
; Raman microspectroscopy
; VBNC
; Viable but non-culturable
; Cells
; chlorine
; drinking water
; tosylchloramide sodium
; bacterium
; cell
; cell component
; chlorination
; concentration (composition)
; disinfection
; drinking water
; environmental stress
; fecal coliform
; gene expression
; generation time
; health risk
; physiology
; virulence
; Article
; cell count
; cell viability
; cells
; chlorination
; confocal microscopy
; controlled study
; Escherichia coli
; health hazard
; morphological trait
; nonhuman
; priority journal
; scanning electron microscopy
; viable but nonculturable cell
; viable cell count
; Bacteria (microorganisms)
; Escherichia coli
; Luria
英文摘要: Many pathogens can enter into a viable but nonculturable (VBNC) state in response to harsh environmental stresses. Bacteria in this state can retain certain features of viable cells, such as cellular integrity, metabolic activity, or virulence and may present health risks associated with drinking water. In this study, we investigated the ability of chlorination and chloramination, which are widely used methods to disinfect drinking water, to induce Escherichia coli into a VBNC state. After treatment with chlorine and chloramine at concentrations of 1, 2, 3, and 4 mg/L, the counts of culturable E. coli cells decreased from 106 CFU/mL to 0 CFU/mL at 5–60 min post treatment. Meanwhile, viable cell counts were still approximately 103–105 cells/mL. These viable E. coli cells may be induced into a VBNC state by chlorination and chloramination. Scanning electron microscopy and laser confocal microscopy showed that some bacteria maintained cellular integrity, but the average length of VBNC cells was less than that of culturable cells. Respiratory activity of VBNC cells decreased approximately 50% relative to that of culturable cells. We also used heavy water (D2O) combined with Raman microspectroscopy to show that E. coli in a VBNC state retained metabolic activity involving water (e.g. condensation reactions) at the single-cell level. Furthermore, soxR, gadA, and katG genes remained highly expressed, suggesting that VBNC cells were physiologically active. Finally, resuscitation of VBNC cells induced by chlorine in Luria-Bertani (LB) broth was identified by calculating the generation time. Results of this study will facilitate a better understanding of the health risks associated with VBNC bacteria and the development of more effective strategies for drinking water disinfection. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112636
Appears in Collections: 气候减缓与适应
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作者单位: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; University of Chinese Academy of Sciences, Beijing, 100049, China; College of Environment & Ecology, Xiamen University, Xiamen, 361021, China
Recommended Citation:
Chen S.,Li X.,Wang Y.,et al. Induction of Escherichia coli into a VBNC state through chlorination/chloramination and differences in characteristics of the bacterium between states[J]. Water Research,2018-01-01,142