DOI: 10.1016/j.watres.2018.01.058
Scopus记录号: 2-s2.0-85041311918
论文题名: Starved viable but non-culturable (VBNC) Legionella strains can infect and replicate in amoebae and human macrophages
作者: Dietersdorfer E. ; Kirschner A. ; Schrammel B. ; Ohradanova-Repic A. ; Stockinger H. ; Sommer R. ; Walochnik J. ; Cervero-Aragó S.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 141 起始页码: 428
结束页码: 438
语种: 英语
英文关键词: Acanthamoeba
; Legionella
; Macrophages
; Starvation
; VBNC
Scopus关键词: Climate change
; Cytology
; Macrophages
; Protozoa
; Waterworks
; Acanthamoeba
; Industrialized countries
; Legionella
; Microbiological cultures
; Starvation
; VBNC
; Viable but non-culturable
; Water borne infections
; Potable water
; colony stimulating factor 1
; granulocyte macrophage colony stimulating factor
; aging population
; bacterium
; biofilm
; biofiltration
; cell component
; detection method
; drinking water
; eukaryote
; infectious disease
; microbiology
; starvation
; waterborne disease
; Acanthamoeba castellanii
; amoeba (life cycle stage)
; Article
; axenic culture
; bacterial viability
; bacterium culture
; coculture
; controlled study
; host cell
; human
; human cell
; Legionella
; Legionella pneumophila
; leukocyte
; macrophage
; monocyte
; nonhuman
; peripheral blood mononuclear cell
; priority journal
; THP-1 cell line
; trophozoite
; amoeba (life cycle stage)
; legionnaire disease
; macrophage
; microbiology
; pathogenicity
; virulence
; water pollutant
; Acanthamoeba
; Bacteria (microorganisms)
; Legionella
; Legionella pneumophila serogroup 1
; Amoeba
; Coculture Techniques
; Humans
; Legionella
; Legionellosis
; Macrophages
; Virulence
; Water Pollutants
英文摘要: Legionella infections are among the most important waterborne infections with constantly increasing numbers of cases in industrialized countries, as a result of aging populations, rising numbers of immunocompromised individuals and increased need for conditioned water due to climate change. Surveillance of water systems is based on microbiological culture-based techniques; however, it has been shown that high percentages of the Legionella populations in water systems are not culturable. In the past two decades, the relevance of such viable but non-culturable (VBNC) legionellae has been controversially discussed, and whether VBNC legionellae can directly infect human macrophages, the primary targets of Legionella infections, remains unclear. In this study, it was demonstrated for the first time that several starved VBNC Legionella strains (four L. pneumophila serogroup 1 strains, a serogroup 6 strain and a L. micdadei strain) can directly infect different types of human macrophages and amoebae even after one year of starvation in ultrapure water. However, under these conditions, the strains caused infection with reduced efficacy, as represented by the lower percentages of infected cells, prolonged time in co-culture and higher multiplicities of infection required. Interestingly, the VBNC cells remained mostly non-culturable even after multiplication within the host cells. Amoebal infection by starved VBNC Legionella, which likely occurs in oligotrophic biofilms, would result in an increase in the bacterial concentration in drinking-water systems. If cells remain in the VBNC state, the real number of active legionellae will be underestimated by the use of culture-based standard techniques. Thus, further quantitative research is needed in order to determine, whether and how many starved VBNC Legionella cells are able to cause disease in humans. © 2018 The Authors Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112657
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: Medical University of Vienna, Institute of Specific Prophylaxis and Tropical Medicine, Department of Medical Parasitology, Kinderspitalgasse 15, Vienna, A-1090, Austria; Medical University of Vienna, Institute for Hygiene and Applied Immunology, Water Hygiene, Kinderspitalgasse 15, Vienna, A-1090, Austria; Interuniversity Cooperation Centre for Water & Health, Vienna, Austria; Medical University of Vienna, Institute for Hygiene and Applied Immunology, Lazarettgasse 19, Vienna, A-1090, Austria
Recommended Citation:
Dietersdorfer E.,Kirschner A.,Schrammel B.,et al. Starved viable but non-culturable (VBNC) Legionella strains can infect and replicate in amoebae and human macrophages[J]. Water Research,2018-01-01,141