DOI: 10.1016/j.watres.2018.04.019
Scopus记录号: 2-s2.0-85052966053
论文题名: Instability diagnosis and syntrophic acetate oxidation during thermophilic digestion of vegetable waste
作者: Li D. ; Ran Y. ; Chen L. ; Cao Q. ; Li Z. ; Liu X.
刊名: Water research
ISSN: 18792448
出版年: 2018
卷: 139 起始页码: 263
结束页码: 271
语种: 英语
英文关键词: Instability diagnosis
; Process monitoring
; Syntrophic acetate oxidization
; Thermophilic digestion
; Vegetable waste
Scopus关键词: acetic acid
; ammonia
; carbon dioxide
; hydrogen
; methane
; volatile fatty acid
; anaerobic growth
; bacterium
; bioreactor
; metabolism
; microbiology
; oxidation reduction reaction
; vegetable
; waste
; Acetic Acid
; Ammonia
; Anaerobiosis
; Bacteria
; Bioreactors
; Carbon Dioxide
; Fatty Acids, Volatile
; Hydrogen
; Methane
; Oxidation-Reduction
; Vegetables
; Waste Products
英文摘要: Effective process monitoring and instability diagnosis are important for stable anaerobic digestion (AD) of vegetable waste (VW). In order to evaluate the performance of thermophilic digestion of VW, to make early diagnosis for instability after organic overload, and to reveal the dynamics of microbial community under different running states, thermophilic AD of VW was carried out under improved organic loading rates (OLR) of 0.5-2.5 g volatile solid (VS)/(L ∙ d) in this study. Gaseous parameters including volumetric methane production rate (VMPR), CH4, CO2, and H2 concentrations, and liquid parameters including pH, oxidation-reduction potential, volatile fatty acid (VFA), and total alkalinity (TA), bicarbonate alkalinity (BA), intermediate alkalinity (IA), and ammonia, were monitored. The coupling parameters, such as the CH4/CO2, VFA/BA, and BA/TA ratios were also used to evaluate stability. The dynamics of syntrophic acetate-oxidizing bacteria (SAOB), acetoclastic methanogens (AM), and hydrogenotrophic methanogens (HM) were analyzed by high-throughput sequencing. The main methanogenic bacteria were HM (Methanothermobacter) during the start-up period of OLR 0.5 gVS/(L ∙ d), while they were AM (Methanosarcina) during the stable period of OLR of 1.0 gVS/(L ∙ d). The VMPR of stable period was about 0.29 L/(L · d) with total VFA concentration below 100 mg/L, CH4/CO2 > 1.3, and BA/TA>0.9. The first instability due to the accumulation of VFA and self-recovery due to syntrophic acetate oxidation occurred at an OLR of 1.5 gVS/(L ∙ d). The syntrophic acetate-oxidizing bacteria probably belong to genus S1 (family Thermotogaceae). The digestion failed at an OLR of 2.0 g VS/(L · d). H2 was only detected during collapsed period instead of instable period. The total ammonia nitrogen loss and bicarbonate alkalinity (BA) reduction were the primary causes for the instability of AD of VW without effluent recirculation. Compared with single parameters, the CH4/CO2 and BA/total alkalinity (TA) ratios are recommended as early warning indicators for engineering applications of thermophilic AD of VW. Copyright © 2018 Elsevier Ltd. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112764
Appears in Collections: 气候减缓与适应
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作者单位: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China; Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China; Institute of Urban and Rural Mines, Changzhou University, Changzhou, 213164, China; Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China
Recommended Citation:
Li D.,Ran Y.,Chen L.,et al. Instability diagnosis and syntrophic acetate oxidation during thermophilic digestion of vegetable waste[J]. Water research,2018-01-01,139