DOI: 10.1016/j.watres.2018.03.024
Scopus记录号: 2-s2.0-85046014560
论文题名: Genotypic characterization and assessment of infectivity of human waterborne pathogens recovered from oysters and estuarine waters in Brazil
作者: Leal D.A.G. ; Souza D.S.M. ; Caumo K.S. ; Fongaro G. ; Panatieri L.F. ; Durigan M. ; Rott M.B. ; Barardi C.R.M. ; Franco R.M.B.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 137 起始页码: 273
结束页码: 280
语种: 英语
英文关键词: Acanthamoeba
; brackish water
; Giardia duodenalis
; Human adenovirus
; Oysters
; Viral infectivity
Scopus关键词: Catchments
; Disinfection
; Estuaries
; Protozoa
; Sewage
; Shellfish
; Viruses
; Water filtration
; Water pollution
; Water treatment
; Acanthamoeba
; Brackish water
; Giardia duodenalis
; Human adenovirus
; Oysters
; Viral infectivity
; Molluscs
; brackish water
; brackish water
; epidemiology
; estuarine environment
; estuarine pollution
; genotype
; infectious disease
; infectivity
; molecular analysis
; mollusc
; pathogen
; waterborne disease
; Acanthamoeba
; Adenoviridae
; Article
; Brazil
; catchment
; estuary
; genotype
; Giardia intestinalis
; human
; Human adenovirus C
; infection
; mollusc
; nonhuman
; oyster
; phylogeny
; priority journal
; water borne disease
; Acanthamoeba
; analysis
; animal
; environmental monitoring
; food contamination
; genetics
; Giardia intestinalis
; isolation and purification
; microbiology
; oyster
; pathogenicity
; water management
; water pollution
; Brazil
; Sao Paulo [Brazil]
; Acanthamoeba
; Adenoviridae
; Giardia
; Giardia intestinalis
; Human adenovirus
; Mollusca
; Ostreidae
; Protozoa
; Acanthamoeba
; Adenoviruses, Human
; Animals
; Brazil
; Environmental Monitoring
; Estuaries
; Food Contamination
; Genotype
; Giardia lamblia
; Humans
; Ostreidae
; Water Pollution
; Water Purification
英文摘要: Waterborne, food-borne and sewage-borne pathogens are a major global concern, with the annual recurrence, most notably during the summer, of outbreaks of gastroenteritis of unconfirmed etiology associated with recreational activities in marine environments. The consumption of contaminated water-based foodstuffs is also related to outbreaks of human illness. The main goals of the present study were: i) to identify the genetic assemblages of Giardia duodenalis cysts in growing and depurated oysters destined for human consumption on the southern coast of São Paulo, Brazil; ii) to verify the main circulating G. duodenalis assemblages and their subtypes in different brackish waters used for the production of mollusks and for recreational purposes; iii) to track the contamination of growing and depurated oysters by the human adenovirus and identify the infectivity of adenoviral particles recovered from oysters before and after depuration; iv) to evaluate the occurrence and genotype of the free-living amoebae of the genus Acanthamoeba in brackish water and oysters from all the sites described above. Four sampling sites in the Cananeia estuary were selected to search for pathogenic and amphizoic protozoa (Giardia and Acanthamoeba respectively): site 1: oyster growth, site 2: catchment water (before UV depuration procedure), site 3: filter backwash (filtration stage of water treatment) and site 4: oyster depuration tank. Oysters at sites 1 and 4 were evaluated for the presence of adenovirus (HAdV). Analysis consisted of conventional microbiological as well as molecular methods. Giardia duodenalis were detected in all the water sites analyzed and the molecular analysis revealed that sub-assemblage AII was the most frequently distributed throughout the estuarine environment, although one sample was identified as belonging to the assemblage C. Acanthamoeba were also isolated from different locations of the estuarine area, and were detected at all the analyzed sites. The majority of isolates belonged to the T3 genotype, while the T4 genotype was identified once. The sequencing reaction of Giardia duodenalis revealed the contamination of three batches of depurated oysters by the sub-assemblage AII. With respect to viruses, seven batches of oysters (four growing and three depurated) were found to be harboring infectious HAdV particles when submitted to plaque assay. Overall, the results of the sequencing reactions combined with the plaque assay revealed that the isolates of Giardia duodenalis and the infectious HAdV particles identified in oyster tissues have the potential to infect humans and pose a threat if consumed raw or lightly cooked. This is the first report on the sub-assemblage AII identified in oysters which are submitted to a cleaning and disinfection procedure prior to human consumption in Brazil. Acanthamoeba specific genotypes were also identified for the first time in a recreational estuarine area in Brazil, contributing to knowledge of their molecular and environmental epidemiology, which is considered scarce even in marine and estuarine areas of the world. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112834
Appears in Collections: 气候减缓与适应
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作者单位: Universidade Federal do Paraná (UFPR), Setor de Ciências Biológicas, Departamento de Patologia Básica, Laboratório de Parasitologia Ambiental, PO Box: 19031, Curitiba, Paraná 81531-980, Brazil; Universidade Federal de Santa Catarina (UFSC), Centro de Ciências Biológicas, Departamento de Microbiologia e Parasitologia, Laboratório de Virologia Aplicada, Florianópolis, Santa Catarina 88040-970, Brazil; Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Estudos de Protozoários Emergentes, Florianópolis, Santa Catarina 88040-970, Brazil; Universidade Federal do Rio Grande do Sul (UFRGS), Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Porto Alegre, Rio Grande do Sul 900035-190, Brazil; U.S. FDA, OARSA, CFSAN, Laurel, MD, United States; Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Biologia Animal, Laboratório de Protozoologia, Campinas, São Paulo 13083-970, Brazil
Recommended Citation:
Leal D.A.G.,Souza D.S.M.,Caumo K.S.,et al. Genotypic characterization and assessment of infectivity of human waterborne pathogens recovered from oysters and estuarine waters in Brazil[J]. Water Research,2018-01-01,137