DOI: 10.1016/j.watres.2018.02.062
Scopus记录号: 2-s2.0-85042733498
论文题名: mcrA sequencing reveals the role of basophilic methanogens in a cathodic methanogenic community
作者: Cai W. ; Liu W. ; Zhang Z. ; Feng K. ; Ren G. ; Pu C. ; Sun H. ; Li J. ; Deng Y. ; Wang A.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 136 起始页码: 192
结束页码: 199
语种: 英语
英文关键词: Bioelectrochemical
; Cathode
; mcrA
; Methanogenic community
; pH-gradient
Scopus关键词: Biofilms
; Cathodes
; pH
; Applied potentials
; Bio-electrochemical
; Hydrogenotrophic methanogens
; mcrA
; Methane production
; Methanogenic community
; Microbial community structures
; pH gradients
; Methanogens
; methane
; archaeal protein
; endodeoxyribonuclease McrA
; restriction endonuclease
; anoxic conditions
; biofilm
; community structure
; electrode
; gene
; gene expression
; genetic analysis
; methanogenic bacterium
; microbial activity
; microbial community
; microbial ecology
; microorganism
; pH
; Article
; bacterial gene
; basophil
; biofilm
; community structure
; ecological niche
; gene sequence
; mcra gene
; Methanobacterium
; Methanobacterium alcaliphilum
; methanogenesis
; methanogenic bacterium
; microbial community
; nonhuman
; pH
; priority journal
; biosynthesis
; enzymology
; genetics
; metabolism
; Methanobacterium
; Archaeal Proteins
; DNA Restriction Enzymes
; Hydrogen-Ion Concentration
; Methane
; Methanobacterium
英文摘要: Cathodic methanogenesis is a promising method for accelerating and stabilising bioenergy recovery in anaerobic processes. The change in composition of microbial (especially methanogenic) communities in response to an applied potential—and especially the associated pH gradient—is critical for achieving this goal, but is not well understood in cathodic biofilms. We found here that the pH-polarised region in the 2 mm surrounding the cathode ranged from 6.9 to 10.1, as determined using a pH microsensor; this substantially affected methane production rate as well as microbial community structure. Miseq sequencing data of a highly conserved region of the mcrA gene revealed a dramatic variation in alpha diversity of methanogens concentrated in electrode biofilms under the applied potential, and confirmed that the dominant microbes at the cathode were hydrogenotrophic methanogens (mostly basophilic Methanobacterium alcaliphilum). These results indicate that regional pH variation in the microenvironment surrounding the electrode is an ecological niche enriched with Methanobacterium. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112854
Appears in Collections: 气候减缓与适应
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作者单位: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China
Recommended Citation:
Cai W.,Liu W.,Zhang Z.,et al. mcrA sequencing reveals the role of basophilic methanogens in a cathodic methanogenic community[J]. Water Research,2018-01-01,136