DOI: 10.1016/j.watres.2018.02.041
Scopus记录号: 2-s2.0-85042514161
论文题名: An improved method for PCR-based detection and routine monitoring of geosmin-producing cyanobacterial blooms
作者: John N. ; Koehler A.V. ; Ansell B.R.E. ; Baker L. ; Crosbie N.D. ; Jex A.R.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 136 起始页码: 34
结束页码: 40
语种: 英语
英文关键词: Cyanobacteria
; Geosmin
; PCR
; Phylogenetic tree
; Sliding window analysis
; Taste and odour
Scopus关键词: Bacteria
; Chemical analysis
; Water quality
; Cyanobacteria
; Geosmin
; Phylogenetic trees
; Sliding-window analysis
; Taste and odour
; Polymerase chain reaction
; geosmin
; nucleotide
; bacterial protein
; flavoring agent
; fragrance
; geosmin
; naphthol derivative
; algal bloom
; cyanobacterium
; detection method
; monitoring system
; numerical method
; odor
; organic compound
; phylogenetics
; polymerase chain reaction
; taste
; water quality
; amplicon
; analytic method
; Article
; Australia
; bacterial overgrowth
; bacterium detection
; chemical analysis
; controlled study
; cyanobacterium
; Dolichospermum ucrainicum
; gene
; gene amplification
; gene sequence
; geoA gene
; monitoring
; nonhuman
; Nostoc punctiforme
; nucleotide sequence
; phylogeny
; polymerase chain reaction
; priority journal
; water management
; water quality
; cyanobacterium
; evaluation study
; genetics
; growth, development and aging
; isolation and purification
; metabolism
; polymerase chain reaction
; procedures
; Victoria
; Australia
; Victoria [Australia]
; Cyanobacteria
; Nostoc punctiforme
; Bacterial Proteins
; Cyanobacteria
; Flavoring Agents
; Naphthols
; Odorants
; Polymerase Chain Reaction
; Victoria
英文摘要: Production of taste and odour (T/O) compounds, principally geosmin, by complex cyanobacterial blooms is a major water quality issue globally. Control of these cyanobacteria imposes a significant cost on water producing and dependent industries, and requires routine monitoring and management. Classic monitoring methods, including microscopy and direct chemical analysis, lack sensitivity, are laborious, expensive or cannot reliably identify the source of geosmin production. Polymerase Chain Reaction (PCR) based tools targeting the geosmin synthase gene (geoA) provide a novel tool for routine monitoring. However, geoA is variable at the nucleotide level and potential geosmin producers represent a broad taxonomic distribution, such that multiple PCR primers with distinct amplification protocols are needed to target all potential sources of this important T/O compound. Development of novel primers is hindered by a lack of sequence data and limited field and laboratory data on geosmin producers prevents prioritizing taxa for PCR testing. Here we performed a genetic screen of 253 bloom samples from Victoria, Australia using each existing PCR protocol targeting geoA. We detected Dolichospermum ucrainicum as the major geosmin producer (87% of sequenced samples) along with 3 unknown geoA sequence types. Using these data, we designed a novel, short amplicon, PCR protocol utilising a single standardised primer pair, capable of amplifying all geoA positive samples in our study, as well as a Nostoc punctiforme positive control. This single protocol geoA PCR can further be tested on other geosmin producers and will simplify routine monitoring of T/O producing cyanobacteria. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112860
Appears in Collections: 气候减缓与适应
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作者单位: Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; Melbourne Water, Docklands, Victoria 3008, Australia
Recommended Citation:
John N.,Koehler A.V.,Ansell B.R.E.,et al. An improved method for PCR-based detection and routine monitoring of geosmin-producing cyanobacterial blooms[J]. Water Research,2018-01-01,136