DOI: 10.1016/j.watres.2018.01.053
Scopus记录号: 2-s2.0-85041666421
论文题名: Impacts of virus processing on human norovirus GI and GII persistence during disinfection of municipal secondary wastewater effluent
作者: Dunkin N. ; Weng S. ; Coulter C.G. ; Jacangelo J.G. ; Schwab K.J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 134 起始页码: 1
结束页码: 12
语种: 英语
英文关键词: Chlorination
; Norovirus
; Stool purification
; Wastewater
; Water treatment
Scopus关键词: Chlorination
; Chlorine
; Disinfection
; Effluents
; Environmental regulations
; Polymerase chain reaction
; Purification
; Solvent extraction
; Viruses
; Wastewater
; Wastewater treatment
; Water filtration
; Water treatment
; Inactivation models
; Norovirus
; Purification process
; Reverse transcription
; Ultracentrifugation
; Wastewater effluents
; Water and wastewater
; Waterborne transmission
; Chemicals removal (water treatment)
; chlorine
; phosphate
; sucrose
; virus RNA
; chlorine
; virus RNA
; chlorination
; chlorine
; disinfection
; effluent
; extraction method
; filtration
; persistence
; pollutant removal
; purification
; reaction kinetics
; reduction
; virus
; wastewater
; water treatment
; Article
; chlorination
; controlled study
; effluent
; feces
; human
; molecular weight
; nonhuman
; Norovirus
; priority journal
; reverse transcription polymerase chain reaction
; size exclusion chromatography
; solvent extraction
; ultracentrifugation
; ultrafiltration
; virus characterization
; virus inactivation
; virus purification
; virus resistance
; virus strain
; waste water management
; water quality
; disinfection
; drug effect
; genetics
; halogenation
; Norovirus
; procedures
; real time polymerase chain reaction
; sewage
; virology
; waste water
; water pollutant
; Norovirus
; Chlorine
; Disinfection
; Halogenation
; Norovirus
; Real-Time Polymerase Chain Reaction
; RNA, Viral
; Waste Disposal, Fluid
; Waste Water
; Water Pollutants
英文摘要: Noroviruses cause significant global health burdens and waterborne transmission is a known exposure pathway. Chlorination is the most common method of disinfection for water and wastewater worldwide. The purpose of this study was to investigate the underlying causes for discrepancies in human norovirus (hNoV) resistance to free chlorine that have been previously published, and to assess hNoV GI and GII persistence during disinfection of municipal secondary wastewater (WW) effluent. Our results reveal that choice of hNoV purification methodology prior to seeding the viruses in an experimental water matrix influences disinfection outcomes in treatment studies. Common hNoV purification processes such as solvent extraction and 0.45-μm filtration were ineffective in removing high levels of organics introduced into water or wastewater samples when seeding norovirus positive stool. These methods resulted in experimental water matrices receiving an additional 190 mg/L as Cl2 of 15-s chlorine demand and approximately 440 mg/L as Cl2 of 30-min chlorine demand due to seeding norovirus positive stool at 1% w/v. These high organic loads impact experimental water chemistry and bias estimations of hNoV persistence. Advanced purification of norovirus positive stool using sucrose cushion ultracentrifugation and ultrafiltration reduced 15-s chlorine demands by 99% and TOC by 93% for loose (i.e. unformed diarrhea) stools. Using these methods, hNoV GI and GII persistence was investigated during free chlorination of municipal WW. A suite five of kinetic inactivation models was fit to viral reverse transcription-qPCR reduction data, and model predicted CT values for 1, 2, and 3 log10 reduction of hNoV GI in municipal WW by free chlorine were 0.3, 2.1, and 7.8 mg-min/L, respectively. Model predicted CT values for reduction of hNoV GII in WW were 0.4, 2.0, and 7.0 mg-min/L, respectively. These results indicate that current WW treatment plant disinfection practices employing free chlorine are likely protective for public health with regards to noroviruses, and will achieve at least 3-log reduction of hNoV GI and GII RNA despite previous reports of high hNoV resistance. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112915
Appears in Collections: 气候减缓与适应
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作者单位: Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States; JHU/MWH Alliance, Johns Hopkins University, Baltimore, MD, United States; MWH-Stantec, Pasadena, CA, United States
Recommended Citation:
Dunkin N.,Weng S.,Coulter C.G.,et al. Impacts of virus processing on human norovirus GI and GII persistence during disinfection of municipal secondary wastewater effluent[J]. Water Research,2018-01-01,134