DOI: 10.1016/j.watres.2018.01.063
Scopus记录号: 2-s2.0-85041453934
论文题名: Antibiotic resistome in landfill leachate from different cities of China deciphered by metagenomic analysis
作者: Zhao R. ; Feng J. ; Yin X. ; Liu J. ; Fu W. ; Berendonk T.U. ; Zhang T. ; Li X. ; Li B.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 134 起始页码: 126
结束页码: 139
语种: 英语
英文关键词: Anaerobic digestion sludge
; Antibiotic resistance genes
; Landfill leachate
; Metagenomic analysis
; Network analysis
Scopus关键词: Anaerobic digestion
; Antibiotics
; Electric network analysis
; Genes
; Reservoir management
; Risk management
; RNA
; Sewage treatment plants
; Sludge digestion
; Antibiotic resistance genes
; Environmental sample
; High-throughput sequencing
; Landfill environment
; Landfill leachates
; Metagenomic analysis
; Microbial community composition
; Regional distribution
; Leachate treatment
; aminoglycoside
; ampicillin
; antibiotic agent
; bacitracin
; beta lactam
; cefalexin
; cefotaxime
; chloramphenicol
; ciprofloxacin
; doxycycline
; enrofloxacin
; erythromycin
; fosmidomycin
; lincomycin
; lincosamide
; macrolide
; norfloxacin
; ofloxacin
; pefloxacin
; polymyxin
; RNA 16S
; roxithromycin
; streptogramin derivative
; sulfadiazine
; sulfadimidine
; sulfamethoxazole
; sulfamonomethoxine
; sulfaquinoxaline
; tetracycline
; trimethoprim
; anoxic conditions
; community composition
; genetic analysis
; genomics
; landfill
; leachate
; microbial community
; network analysis
; risk assessment
; sludge
; aada gene
; anaerobic digestion
; antibiotic resistance
; antibiotic resistance gene
; Article
; baca gene
; chemical parameters
; China
; city
; controlled study
; environmental factor
; gene
; genetic analysis
; genetic resistance
; geographic distribution
; high throughput sequencing
; human
; incidence
; landfill leachate
; metagenomics
; microbial community
; municipal solid waste
; priority journal
; quantitative analysis
; risk management
; sewage treatment plant
; sludge digestion
; sul1 gene
; sul2 gene
; analysis
; antibiotic resistance
; bacterial gene
; environmental monitoring
; genetics
; metagenomics
; sewage
; waste disposal facility
; water pollutant
; China
; China
; Cities
; Drug Resistance, Microbial
; Environmental Monitoring
; Genes, Bacterial
; Metagenomics
; RNA, Ribosomal, 16S
; Sewage
; Waste Disposal Facilities
; Water Pollutants, Chemical
英文摘要: High throughput sequencing-based metagenomic analysis and network analysis were applied to investigate the broad-spectrum profiles of ARGs in landfill leachate from 12 cities in China. In total, 526 ARG subtypes belonging to 21 ARG types were detected with abundances ranging from 1.1 × 10−6 to 2.09 × 10−1 copy of ARG/copy of 16S rRNA gene. 68 ARG subtypes that accounted for 73.4%–93.4% of the total ARG abundances were shared by all leachate samples. The four most abundant ARGs, sul1, sul2, aadA and bacA can be served as ARG indicators to quantitatively predict the total abundances by linear functions (r2 = 0.577–0.819, P < 0.001). No distinct regional distribution pattern of the ARGs was observed among different cities in China, while the ARG compositions of the leachate were clearly distinct from those of other environmental sample types. Nearly 90% ARG subtypes in the anaerobic digestion sludge from sewage treatment plants (STPADS) were shared by the leachate and the abundances of leachate and STPADS ARGs generalists accounted for 84.5% and 87.7% of total abundances in these two types of anaerobic samples, respectively. Furthermore, Procrustes analysis suggested that microbial community composition might be the determining factor of ARG compositions in landfill leachate. ARGs within the same type or among the different types showed higher incidences of non-random co-occurrence and 17 genera might be potential hosts of multiple ARGs. This study highlighted that landfill leachate is an important reservoir of various ARGs and provided a useful reference for the surveillance and risk management of ARGs in landfill environments. © 2018 Elsevier Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112918
Appears in Collections: 气候减缓与适应
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作者单位: Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China; School of Environment, Tsinghua University, Beijing, 100084, China; Environmental Biotechnology Laboratory, The University of Hong KongHong Kong, China; Institute for Hydrobiology, Technische Universität Dresden, Germany
Recommended Citation:
Zhao R.,Feng J.,Yin X.,et al. Antibiotic resistome in landfill leachate from different cities of China deciphered by metagenomic analysis[J]. Water Research,2018-01-01,134