DOI: 10.1016/j.watres.2017.12.011
Scopus记录号: 2-s2.0-85039717939
论文题名: Evaluation of the novel crAssphage marker for sewage pollution tracking in storm drain outfalls in Tampa, Florida
作者: Ahmed W. ; Lobos A. ; Senkbeil J. ; Peraud J. ; Gallard J. ; Harwood V.J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 131 起始页码: 142
结束页码: 150
语种: 英语
英文关键词: crAssphage
; Fecal indicator bacteria
; Fecal pollution
; Microbial source tracking
; Stormwater
Scopus关键词: Bacteriophages
; Contamination
; DNA
; Genes
; Meteorology
; Outfalls
; Pollution
; Polymerase chain reaction
; River pollution
; Sewage
; Storm sewers
; Storms
; Surface waters
; crAssphage
; Fecal indicator bacteria
; Fecal pollution
; Microbial source tracking
; Stormwaters
; Water pollution
; river water
; storm water
; surface water
; bacteriophage
; bioindicator
; biomarker
; concentration (composition)
; drain
; fecal coliform
; performance assessment
; sewage
; sewage outfall
; subtropical region
; tracking
; wastewater
; Article
; bacteriophage
; Bacteroides
; controlled study
; crassphage
; cross reaction
; DNA extraction
; Enterococcus
; Escherichia coli
; Florida
; human
; limit of detection
; limit of quantitation
; nonhuman
; pollution
; polymerase chain reaction
; poultry
; priority journal
; quantitative analysis
; sensitivity analysis
; sewage
; weather
; Florida [United States]
; Tampa
; United States
; Bacteroides
英文摘要: CrAssphage are recently-discovered DNA bacteriophages that are prevalent and abundant in human feces and sewage. We assessed the performance characteristics of a crAssphage quantitative PCR (qPCR) assay for quantifying sewage impacts in stormwater and surface water in subtropical Tampa, Florida. The mean concentrations of crAssphage in untreated sewage ranged from 9.08 to 9.98 log10 gene copies/L. Specificity was 0.927 against 83 non-human fecal reference samples and the sensitivity was 1.0. Cross-reactivity was observed in DNA extracted from soiled poultry litter but the concentrations were substantially lower than untreated sewage. The presence of the crAssphage marker was monitored in water samples from storm drain outfalls during dry and wet weather conditions in Tampa, Florida. In dry weather conditions, 41.6% of storm drain outfalls samples were positive for the crAssphage marker and the concentrations ranged from 3.60 to 4.65 log10 gene copies/L of water. After a significant rain event, 66.6% of stormwater outlet samples were positive for the crAssphage marker and the concentration ranged from 3.62 to 4.91 log10 gene copies/L of water. The presence of the most commonly used Bacteroides HF183 marker in storm drain outfalls was also tested along with the crAssphage. Thirteen samples (55%) were either positive (i.e., both markers were present) or negative (i.e., both markers were absent) for both the markers. Due to the observed cross-reactivity of this marker with DNA extracted from poultry litter samples, it is recommended that this marker should be used in conjunction with additional markers such as HF183. Our data indicate that the crAssphage marker is highly sensitive to sewage, is adequately specific, and will be a valuable addition to the MST toolbox. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113002
Appears in Collections: 气候减缓与适应
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作者单位: CSIRO Land and Water, Ecosciences Precinct, 41 Boggo RoadQld 4102, Australia; Department of Integrative Biology, SCA 110, University of South Florida, 4202 East Fowler Ave, Tampa, FL 33620, United States
Recommended Citation:
Ahmed W.,Lobos A.,Senkbeil J.,et al. Evaluation of the novel crAssphage marker for sewage pollution tracking in storm drain outfalls in Tampa, Florida[J]. Water Research,2018-01-01,131