DOI: 10.1016/j.watres.2017.12.023
Scopus记录号: 2-s2.0-85042233003
论文题名: Portable platform for rapid in-field identification of human fecal pollution in water
作者: Jiang Y.S. ; Riedel T.E. ; Popoola J.A. ; Morrow B.R. ; Cai S. ; Ellington A.D. ; Bhadra S.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 131 起始页码: 186
结束页码: 195
语种: 英语
英文关键词: DNA strand displacement probes
; Fecal indicator bacteria
; Fecal source identification
; Isothermal nucleic acid amplification
; Portable molecular diagnostics
Scopus关键词: 3D printers
; Assays
; Contamination
; Health risks
; Isotherms
; Nucleic acids
; Oligonucleotides
; Polymerase chain reaction
; Probes
; Sewage
; Dna strand displacements
; Environmental water
; Fecal indicator bacteria
; Fecal sources
; Human fecal pollution
; Loop mediated isothermal amplifications
; Molecular diagnostics
; Sample preparation methods
; Water pollution
; DNA polymerase
; nucleic acid
; oligonucleotide
; sea water
; water
; Article
; bacterium culture
; Bacteroides
; cost
; cross reaction
; Enterococcus
; Escherichia coli
; false positive result
; feces
; feces analysis
; feces microflora
; freeze drying
; genetic marker
; human
; limit of detection
; loop mediated isothermal amplification
; polymerase chain reaction
; priority journal
; public health
; risk
; sequence analysis
; sewage treatment
; water contamination
; water pollution
; water quality
; water supply
; Bacteria (microorganisms)
; Bacteroides
; Felidae
英文摘要: Human fecal contamination of water is a public health risk. However, inadequate testing solutions frustrate timely, actionable monitoring. Bacterial culture-based methods are simple but typically cannot distinguish fecal host source. PCR assays can identify host sources but require expertise and infrastructure. To bridge this gap we have developed a field-ready nucleic acid diagnostic platform and rapid sample preparation methods that enable on-site identification of human fecal contamination within 80 min of sampling. Our platform relies on loop-mediated isothermal amplification (LAMP) of human-associated Bacteroides HF183 genetic markers from crude samples. Oligonucleotide strand exchange (OSD) probes reduce false positives by sequence specifically transducing LAMP amplicons into visible fluorescence that can be photographed by unmodified smartphones. Our assay can detect as few as 17 copies/ml of human-associated HF183 targets in sewage-contaminated water without cross-reaction with canine or feline feces. It performs robustly with a variety of environmental water sources and with raw sewage. We have also developed lyophilized assays and inexpensive 3D-printed devices to minimize cost and facilitate field application. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113030
Appears in Collections: 气候减缓与适应
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作者单位: Department of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, United States; Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, United States; Brigham and Women's hospital, Harvard Medical School, Boston, MA 02115, United States; Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang 310058, China
Recommended Citation:
Jiang Y.S.,Riedel T.E.,Popoola J.A.,et al. Portable platform for rapid in-field identification of human fecal pollution in water[J]. Water Research,2018-01-01,131