DOI: 10.1016/j.watres.2017.11.068
Scopus记录号: 2-s2.0-85036670787
论文题名: Spatial and temporal dynamics of pathogenic Leptospira in surface waters from the urban slum environment
作者: Casanovas-Massana A. ; Costa F. ; Riediger I.N. ; Cunha M. ; de Oliveira D. ; Mota D.C. ; Sousa E. ; Querino V.A. ; Nery N. ; Jr. ; Reis M.G. ; Wunder E.A. ; Jr. ; Diggle P.J. ; Ko A.I.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 130 起始页码: 176
结束页码: 184
语种: 英语
英文关键词: Leptospira
; Leptospirosis
; Public health
; Sewage
; Surface water
; Urban slum
Scopus关键词: DNA
; Dynamics
; Housing
; Polymerase chain reaction
; Public health
; Sewage
; Leptospira
; Leptospirosis
; Linear mixed models
; Quantitative PCR
; Sampling schemes
; Spatial and temporal heterogeneity
; Temporal dynamics
; Urban slum
; Surface waters
; bacterial DNA
; rain
; surface water
; bacterium
; disease incidence
; disease transmission
; DNA
; dry season
; health risk
; leptospirosis
; pathogen
; public health
; sewage
; spatiotemporal analysis
; surface water
; urban ecosystem
; Article
; Brazilian
; controlled study
; dynamics
; environmental factor
; epidemic
; Leptospira
; leptospirosis
; nonhuman
; polymerase chain reaction
; priority journal
; quantitative analysis
; season
; sewage
; urban area
; water sampling
; Brazil
; human
; isolation and purification
; Leptospira
; leptospirosis
; microbiology
; poverty
; statistical model
; Brazil
; Leptospira
; Brazil
; Humans
; Leptospira
; Leptospirosis
; Linear Models
; Logistic Models
; Poverty Areas
; Rain
; Seasons
; Sewage
; Water Microbiology
英文摘要: Leptospirosis has emerged as an important urban health problem as slum settlements have expanded worldwide. Yet the dynamics of the environmentally transmitted Leptospira pathogen has not been well characterized in these settings. We used a stratified dense sampling scheme to study the dynamics of Leptospira abundance in surface waters from a Brazilian urban slum community. We collected surface water samples during the dry, intermediate and rainy seasons within a seven-month period and quantified pathogenic Leptospira by quantitative PCR (qPCR). We used logistic and linear mixed models to identify factors that explained variation for the presence and concentration of Leptospira DNA. Among 335 sewage and 250 standing water samples, Leptospira DNA were detected in 36% and 34%, respectively. Among the 236 samples with positive results geometric mean Leptospira concentrations were 152 GEq/mL. The probability of finding Leptospira DNA was higher in sewage samples collected during the rainy season when increased leptospirosis incidence occurred, than during the dry season (47.2% vs 12.5%, respectively, p = 0.0002). There was a marked spatial and temporal heterogeneity in Leptospira DNA distribution, for which type of water, elevation, and time of day that samples were collected, in addition to season, were significant predictors. Together, these findings indicate that Leptospira are ubiquitous in the slum environment and that the water-related risk to which inhabitants are exposed is low. Seasonal increases in Leptospira presence may explain the timing of leptospirosis outbreaks. Effective prevention will need to consider the spatial and temporal dynamics of pathogenic Leptospira in surface waters to reduce the burden of the disease. © 2017 Elsevier Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113070
Appears in Collections: 气候减缓与适应
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作者单位: Department of Epidemiology of Microbial Diseases, School of Public Health, Yale University, 60 College Street, New Haven, CT, United States; Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Saúde, Rua Waldemar Falcão, 121, Salvador, Bahia, Brazil; Instituto de Saúde Coletiva, Universidade Federal da Bahia, Rua Basílio da Gama, s/n, Campus Universitário Canela, Salvador, Bahia, Brazil; Laboratório Central do Estado do Paraná, Curitiba, Paraná, Brazil, Rua Sebastiana Santana Fraga, 1001, Guatupê, São José dos Pinhais, Paraná, Brazil; Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rua Leopoldo Bulhões, 1480 - Manguinhos, Rio de Janeiro, Brazil; Faculty of Health and Medicine, University of Lancaster, Lancaster, LA1 4YG, United Kingdom
Recommended Citation:
Casanovas-Massana A.,Costa F.,Riediger I.N.,et al. Spatial and temporal dynamics of pathogenic Leptospira in surface waters from the urban slum environment[J]. Water Research,2018-01-01,130