DOI: 10.1016/j.watres.2017.11.014
Scopus记录号: 2-s2.0-85034444819
论文题名: Effect of TiO2 and CeO2 nanoparticles on the metabolic activity of surficial sediment microbial communities based on oxygen microelectrodes and high-throughput sequencing
作者: Miao L. ; Wang P. ; Wang C. ; Hou J. ; Yao Y. ; Liu J. ; Lv B. ; Yang Y. ; You G. ; Xu Y. ; Liu Z. ; Liu S.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 129 起始页码: 287
结束页码: 296
语种: 英语
英文关键词: Bacterial diversity
; Metabolic activity
; Metallic oxide nanoparticles
; Oxygen consumption
; Surficial sediments
Scopus关键词: Aquatic ecosystems
; Bacteria
; Biogeochemistry
; Cerium compounds
; Ecology
; Electrodes
; Enzyme activity
; Metabolism
; Metallic compounds
; Metals
; Microelectrodes
; Microorganisms
; Nanoparticles
; Oxides
; Oxygen
; Sediments
; Throughput
; Titanium compounds
; Titanium dioxide
; Waste treatment
; Water
; Bacterial community structure
; Bacterial diversity
; Biogeochemical conditions
; High-throughput sequencing
; Metabolic activity
; Metallic oxides
; Oxygen consumption
; Potential ecological risk
; Surficial sediments
; brackish water
; cerium oxide nanoparticle
; fresh water
; RNA 16S
; titanium dioxide nanoparticle
; ceric oxide
; cerium
; metal nanoparticle
; oxygen
; titanium
; titanium dioxide
; aquatic ecosystem
; bacterium
; biogeochemistry
; community structure
; electrode
; environmental risk
; enzyme
; enzyme activity
; estuarine sediment
; freshwater sediment
; genetic analysis
; inorganic compound
; metabolism
; microbial activity
; microbial community
; nanoparticle
; oxygen consumption
; sediment-water interface
; species diversity
; surficial sediment
; toxicity
; toxicity test
; acute toxicity
; aerobic bacterium
; Article
; biogeochemical cycling
; cyanobacterium
; Cytophagceae
; enzyme activity
; high throughput sequencing
; Methylotenera
; microbial community
; microbial metabolism
; microcosm
; nonhuman
; oxygen concentration
; oxygen consumption
; polymerase chain reaction
; priority journal
; quantitative analysis
; sediment
; sedimentation
; steady state
; bacterium
; drug effect
; ecosystem
; genetics
; high throughput sequencing
; microbiology
; microelectrode
; microflora
; sediment
; Bacteria (microorganisms)
; Cyanobacteria
; Methylotenera
; Bacteria
; Cerium
; Ecosystem
; Fresh Water
; Geologic Sediments
; High-Throughput Nucleotide Sequencing
; Metal Nanoparticles
; Microbiota
; Microelectrodes
; Oxygen
; Titanium
; Water Microbiology
英文摘要: Environmental concerns regarding the potential ecological risks of metallic oxide nanoparticles (MNPs) in aquatic ecosystems are increasing; sediment is considered a sink for these MNPs. Although several studies have studied the potential impact of MNPs on microbial communities in freshwater and estuarine sediments, limited information is available regarding the influence of MNPs on the metabolic activity of surficial sediment microbial communities and related biogeochemical conditions. To address these issues, a microcosm approach was established to study the metabolic response of surficial sediment microbial communities to a single addition of TiO2 or CeO2 NPs (5 mg/L) using oxygen microelectrodes, enzyme activity measurements, and high-throughput sequencing. Rapid sedimentation of MNPs (regardless of NP type) was observed in freshwater samples, and most (up to 85%) accumulated in surface sediments (<5 mm). Microelectrode profile measurements in pre-incubated sediments treated with MNPs showed that the oxygen concentration decreased at a slower rate with increasing sediment depth compared to that in untreated controls. Biological oxygen consumption in the uppermost sediment layer (0–1500 μm) was significantly inhibited by MNPs, as calculated from steady-state microprofiles, with CeO2 NPs resulting in enhanced acute toxicity than TiO2 NPs. High-throughput sequencing showed that MNP exposure increased the bacterial diversity and altered the bacterial community structure, regardless of NP type. The abundance of three dominant bacterial genera, Methylotenera, Cytophagceae_uncultured (classified as an aerobic bacterium), and Cyanobacteria_norank (a facultative bacterium), was markedly reduced by MNPs, which was primarily responsible for inhibiting microbial-mediated oxygen consumption in surficial sediments. In summary, short-term exposure to MNPs negatively affected the metabolic activity of benthic microbial communities, which could influence the biogeochemical functions along the sediment-water interface. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113111
Appears in Collections: 气候减缓与适应
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作者单位: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing, 210098, China; State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-sen University, Guangzhou, 510275, China
Recommended Citation:
Miao L.,Wang P.,Wang C.,et al. Effect of TiO2 and CeO2 nanoparticles on the metabolic activity of surficial sediment microbial communities based on oxygen microelectrodes and high-throughput sequencing[J]. Water Research,2018-01-01,129