gchange  > 气候减缓与适应
DOI: 10.1016/j.watres.2017.10.041
Scopus ID: 2-s2.0-85032665916
Title:
Correlation between E. coli levels and the presence of foodborne pathogens in surface irrigation water: Establishment of a sampling program
Author: Truchado P.; Hernandez N.; Gil M.I.; Ivanek R.; Allende A.
Source Publication: Water Research
ISSN: 431354
Indexed By: SCI ; SCI-E ; EI
Publishing Year: 2018
Volume: 128
pages begin: 226
pages end: 233
Language: 英语
Keyword: Agricultural water ; Fresh produce ; Indicator microorganisms ; Microbiological risk ; PMA-QPCR ; Sampling program ; Viable but non cultivable bacteria
Scopus Keyword: Escherichia coli ; Irrigation ; Polymerase chain reaction ; Reservoirs (water) ; Surface waters ; Water ; Water pollution ; Agricultural water ; Cultivable bacteria ; Fresh produce ; Microbiological risks ; PMA-QPCR ; Food microbiology ; bacterial DNA ; genomic DNA ; propidium monoazide ; reagent ; surface water ; unclassified drug ; water ; bacterium ; bioindicator ; correlation ; irrigation ; microorganism ; pathogen ; persistence ; polymerase chain reaction ; quantitative analysis ; reservoir ; risk ; sampling ; temperature ; Article ; bacterial count ; bacterial gene ; bacterial strain ; bacterial virulence ; bacterium contamination ; climate ; colony forming unit ; comparative study ; concentration (parameters) ; correlational study ; eae gene ; ehxa gene ; environmental temperature ; Escherichia coli ; Escherichia coli O157 ; evaluation study ; irrigation (agriculture) ; limit of detection ; limit of quantitation ; multiplex polymerase chain reaction ; nonhuman ; plate count ; prevalence ; priority journal ; quantitative analysis ; Salmonella ; Salmonella food poisoning ; sensitivity and specificity ; Shiga toxin producing Escherichia coli ; strain difference ; stx1 gene ; stx2 gene ; water contamination ; water quality ; water sampling ; water supply ; bacterium ; environmental monitoring ; irrigation (agriculture) ; isolation and purification ; microbiology ; procedures ; Salmonella ; Bacteria (microorganisms) ; Escherichia coli ; Salmonella ; Agricultural Irrigation ; Bacteria ; Environmental Monitoring ; Escherichia coli O157 ; Salmonella ; Shiga-Toxigenic Escherichia coli ; Water ; Water Microbiology ; Water Quality
English Abstract: To establish the association between microbial indicators and the presence of foodborne pathogens in irrigation water, Escherichia coli was enumerated using two quantification methods (plate counts and PMA-qPCR) and presence/absence of pathogenic microorganisms, including five strains from the Shiga toxigenic E. coli (O157:H7, O26, O103, O111 and O145) and Salmonella spp. were evaluated. The results confirmed that surface water can be considered a microbial hazard when used for irrigation. The levels of viable E. coli were very similar to those of cultivable E. coli, except for irrigation water obtained from water reservoirs. Comparison between the E. coli counts in samples positive and negative for the presence of pathogenic bacteria for the evaluated water sources identified E. coli level of 2.35 log cfu/100 mL as a cut-off able to correctly predict positive and negative samples with 93% sensitivity and 66% specificity, respectively. Thus, for the samples with levels of E. coli under 2.35 log cfu/100 mL (e.g., 2.24 log cfu/100 mL) there was a 90% probability that the samples were not contaminated with pathogenic microorganism in locations with similar prevalence. E. coli levels in irrigation water were affected by the ambient temperature confirming that water source and climate conditions should be taken into account by growers when designing a sampling program and the frequency of the monitoring to make a better and more efficient use of their resources. © 2017 Elsevier Ltd
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Document Type: 期刊论文
Identifier: http://119.78.100.177/globalchange/handle/2HF3EXSE/113143
Appears in Collections:气候减缓与适应

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Affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, CEBAS-CSIC, Campus Universitario de Espinardo, 25, Murcia, 30100, Spain; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NW, United States

Recommended Citation:
Truchado P.,Hernandez N.,Gil M.I.,et al. Correlation between E. coli levels and the presence of foodborne pathogens in surface irrigation water: Establishment of a sampling program[J]. Water Research,2018-01-01,128
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