DOI: 10.1002/2017JD027307
Scopus记录号: 2-s2.0-85041826197
论文题名: Effects of Ice Nucleation Protein Repeat Number and Oligomerization Level on Ice Nucleation Activity
作者: Ling M.L. ; Wex H. ; Grawe S. ; Jakobsson J. ; Löndahl J. ; Hartmann S. ; Finster K. ; Boesen T. ; Šantl-Temkiv T.
刊名: Journal of Geophysical Research: Atmospheres
ISSN: 2169897X
出版年: 2018
卷: 123, 期: 3 起始页码: 1802
结束页码: 1810
语种: 英语
英文关键词: bioaerosols
; precipitation
Scopus关键词: Bacteria (microorganisms)
; Pseudomonas syringae
英文摘要: Ice nucleation active bacteria have attracted particular attention due to their unique ability to produce specific ice nucleation proteins (INpros), which are the most efficient ice nuclei known as they induce nucleation at temperatures close to 0°C. Our model bacterium Pseudomonas syringae strain R10.79 produced INpros containing 67 tandem repeats, forming the proposed ice-binding surface. To understand the role of the INpro repeats as well as the role of intermolecular interactions between INpros for their ice nucleation behavior, we produced a truncated version of the protein with only 16 tandem repeats (INpro16R). The purified INpro16R produced oligomers of varying sizes. Immersion freezing ice nucleation behavior of purified INpro16R was characterized by droplet-freezing assays and in the Leipzig Aerosol Cloud Interaction Simulator. Predominant INpro16R oligomers introduced into Leipzig Aerosol Cloud Interaction Simulator as single particles with diameters of 50 nm or 70 nm were ice nucleation active at temperatures of −26°C and −24°C, respectively. These are much lower temperatures compared to that of intact INpros (−12°C). The data clearly indicated that the number of repeats determines the ice nucleation temperature. In addition, ice nucleation between −9°C and −10°C, comparable to the activity of intact INpro, was caused by higher-order INpro16R oligomers. This supported previous observations that INpro oligomerization increases the ice-binding surface, thereby affecting ice nucleation activity. In conclusion, both repeat number and oligomerization contribute in a seemingly independent manner to the nucleation mechanism of INpros. ©2018. American Geophysical Union. All Rights Reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/114481
Appears in Collections: 气候减缓与适应
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作者单位: Stellar Astrophysics Centre, Department of Physics and Astronomy, Aarhus University, Aarhus, Denmark; Department of Bioscience, Microbiology Section, Aarhus University, Aarhus, Denmark; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark; Institute for Tropospheric Research, Leipzig, Germany; Department of Design Sciences, Lund University, Lund, Sweden; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark
Recommended Citation:
Ling M.L.,Wex H.,Grawe S.,et al. Effects of Ice Nucleation Protein Repeat Number and Oligomerization Level on Ice Nucleation Activity[J]. Journal of Geophysical Research: Atmospheres,2018-01-01,123(3)