| DOI: | 10.1016/j.jcou.2019.02.016
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| Scopus记录号: | 2-s2.0-85062706236
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| 论文题名: | ScCO 2 -foamed silk fibroin aerogel/poly(ϵ-caprolactone) scaffolds containing dexamethasone for bone regeneration |
| 作者: | Goimil L.; Santos-Rosales V.; Delgado A.; Évora C.; Reyes R.; Lozano-Pérez A.A.; Aznar-Cervantes S.D.; Cenis J.L.; Gómez-Amoza J.L.; Concheiro A.; Alvarez-Lorenzo C.; García-González C.A.
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| 刊名: | Journal of CO2 Utilization
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| ISSN: | 22129820
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| 出版年: | 2019
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| 卷: | 31 | | 起始页码: | 51
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| 结束页码: | 64
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| 语种: | 英语
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| 英文关键词: | Dexamethasone
; In vivo bone repair
; Scaffolds
; Silk fibroin aerogel
; Supercritical foaming
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| Scopus关键词: | Aerogels
; Bone
; Cell adhesion
; Polymer blends
; Pore size
; Repair
; Scaffolds
; Tissue
; Tissue regeneration
; Bone repair
; Dexamethasones
; Mercury intrusion porosimetry
; Morphological properties
; Osteogenic differentiation
; Silk fibroin
; Submicron-sized particles
; Supercritical foaming
; Scaffolds (biology)
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| 英文摘要: | Bone scaffolds prepared with porogens and bioactive agents can accelerate bone tissue formation by providing a suitable 3D-porous structure that promotes cell colonization and differentiation towards the osteogenic lineage. In this work, scaffolds containing poly(ϵ-caprolactone) (PCL) as biopolymeric matrix, silk fibroin as cell adhesion promoter, and dexamethasone as osteogenic differentiation agent, were prepared by supercritical foaming (37 °C, 140 bar, 1 h), a solvent-free method providing a straightforward and effective route for the processing of bioactive grafts. Silk fibroin aerogels in the form of submicron-sized particles were herein developed for the first time and evaluated as porosity inducers. These aerogels incorporated in the scaffolds refined the porous structure and facilitated cell infiltration and the biological fluid transport. Dexamethasone was used in two different forms (base, DX and phosphate salt, DS) to unveil their role in bone regeneration. The morphology of the scaffolds was evaluated using mercury intrusion porosimetry, helium pycnometry and in silico structure modelling. Different release profiles were recorded when using DX or DS. The biological performance was assessed in in vivo tests in calvarial defects using a rat model. Results unveiled the interesting morphological properties of the scaffolds in terms of porosity, pore size distribution and interconnectivity, which are compatible with their application in bone repair. In vivo tests showed the importance of the dexamethasone form and release profile in promoting the bone tissue regeneration with a significant increase in the number of ossification foci and in bone repair extent 14 weeks post-implantation for certain formulations. © 2019 Elsevier Ltd. All rights reserved. |
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| 资源类型: | 期刊论文
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/117280
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| Appears in Collections: | 气候变化与战略
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| Recommended Citation: | |
Goimil L.,Santos-Rosales V.,Delgado A.,et al. ScCO 2 -foamed silk fibroin aerogel/poly(ϵ-caprolactone) scaffolds containing dexamethasone for bone regeneration[J]. Journal of CO2 Utilization,2019-01-01,31
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