DOI: 10.1016/j.jcou.2019.01.008
Scopus记录号: 2-s2.0-85061054809
论文题名: Continuous fractionation of whey protein isolates by using supercritical carbon dioxide
作者: Lima J.C. ; Seixas F.A.V. ; Coimbra J.S.R. ; Pimentel T.C. ; Barão C.E. ; Cardozo-Filho L.
刊名: Journal of CO2 Utilization
ISSN: 22129820
出版年: 2019
卷: 30 起始页码: 112
结束页码: 122
语种: 英语
英文关键词: Continuous reactor
; Supercritical carbon dioxide
; Whey protein isolate
; α-Lactalbumin
; β-Lactoglobulin
Scopus关键词: Carbon dioxide
; Dichroism
; High performance liquid chromatography
; Lanthanum compounds
; Proteins
; Alpha-lactalbumin
; Beta-lactoglobulin
; Continuous flow reactors
; Continuous reactors
; Lactoglobulin
; Pressure and temperature
; Supercritical carbon dioxides
; Whey protein isolate
; Supercritical fluid extraction
英文摘要: α-Lactalbumin (α-La) and β-Lactoglobulin (β-Lg) proteins were fractionated from whey protein isolate (WPI) in a continuous flow reactor by using supercritical carbon dioxide (scCO 2 ) as a solvent. Pressure and temperature conditions tested ranged from 8 to 24 MPa and 55 to 65 °C, respectively. The levels of α-La and β-Lg were quantified by High Performance Liquid Chromatography (HPLC). The denaturation profile of the protein fractions was evaluated by circular dichroism (CD). The initial ratio of α-La:β-Lg in the WPI used was 1:2.7. The pressure - temperature (P - T) combination 8 MPa/55 °C was found to be the best condition to obtain α-La, due to the high concentration of α-La (α/β = 3.84) and yield of precipitate (20.9%). The 8 MPa/55 °C condition also generated the β-Lg fraction with high selectivity (α/β = 0.12). In the most extreme pressure/temperature conditions (24 MPa/65 °C), CD results indicated that α-La was partially denatured and β-Lg remained in its native state. © 2019 Elsevier Ltd. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/117294
Appears in Collections: 气候变化与战略
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Recommended Citation:
Lima J.C.,Seixas F.A.V.,Coimbra J.S.R.,et al. Continuous fractionation of whey protein isolates by using supercritical carbon dioxide[J]. Journal of CO2 Utilization,2019-01-01,30