DOI: 10.1016/j.watres.2019.02.040
Scopus记录号: 2-s2.0-85062476597
论文题名: Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge
作者: de Graaff D.R. ; Felz S. ; Neu T.R. ; Pronk M. ; van Loosdrecht M.C.M. ; Lin Y.
刊名: Water Research
ISSN: 431354
出版年: 2019
卷: 155 起始页码: 343
结束页码: 351
语种: 英语
英文关键词: Aerobic granular sludge
; EPS
; Glycoproteins
; Neuraminic acid
; Salinity
; Seawater
; Sialic acids
英文摘要: Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases. In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done. Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix. Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter. FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars. Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains. This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge. © 2019 The Author(s) Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/121890
Appears in Collections: 气候变化事实与影响
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作者单位: Department of Biotechnology, Delft University of Technology, van der Maasweg 9, Delft, HZ 2629, Netherlands; Microbiology of Interfaces, Department River Ecology, Helmholtz Centre for Environmental Research - UFZ, Brueckstrasse 3A, Magdeburg, 39114, Germany
Recommended Citation:
de Graaff D.R.,Felz S.,Neu T.R.,et al. Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge[J]. Water Research,2019-01-01,155