globalchange  > 气候变化事实与影响
DOI: 10.1016/j.watres.2019.02.020
Scopus记录号: 2-s2.0-85062455249
论文题名:
Genome-centric metagenomics resolves microbial diversity and prevalent truncated denitrification pathways in a denitrifying PAO-enriched bioprocess
作者: Gao H.; Mao Y.; Zhao X.; Liu W.-T.; Zhang T.; Wells G.
刊名: Water Research
ISSN: 431354
出版年: 2019
卷: 155
起始页码: 275
结束页码: 287
语种: 英语
英文关键词: Denitrification ; Denitrifying PAO ; Enhanced biological phosphorus removal (EBPR) ; Metagenome-assembled genomes (MAGs) ; Nitrous oxide
英文摘要: Denitrification is the stepwise microbial reduction of nitrate or nitrite (NO 2 − ) to nitrogen gas via the obligate intermediates nitric oxide (NO) and nitrous oxide (N 2 O). Substantial N 2 O accumulation has been reported in denitrifying enhanced biological phosphorus removal (EBPR) bioreactors enriched in denitrifying polyphosphate accumulating organisms (DPAOs), but little is known about underlying mechanisms for N 2 O generation, prevalence of complete versus truncated denitrification pathways, or the impact of NO 2 − feed on DPAO-enriched consortia. To address this knowledge gap, we employed genome-resolved metagenomics to investigate nitrogen transformation potential in a NO 2 − fed denitrifying EBPR bioreactor enriched in Candidatus Accumulibacter and prone to N 2 O accumulation. Our analysis yielded 41 near-complete metagenome-assembled genomes (MAGs), including two co-occurring Accumulibacter strains affiliated with clades IA and IC (the first published genome from this clade) and 39 non-PAO flanking bacterial genomes. The dominant Accumulibacter clade IA encoded genes for complete denitrification, while the lower abundance Accumulibacter clade IC harbored all denitrification genes except for a canonical respiratory NO reductase. Analysis of the 39 non-PAO MAGs revealed a high prevalence of taxa harboring an incomplete denitrification pathway. Of the 27 MAGs harboring capacity for at least one step in the denitrification pathway, 10 were putative N 2 O producers lacking N 2 O reductase, 16 were putative N 2 O reducers that lacked at least one upstream denitrification gene, and only one harbored a complete denitrification pathway. We also documented increasing abundance over the course of reactor operation of putative N 2 O producers. Our results suggest that the unusually high levels of N 2 O production observed in this Accumulibacter-enriched consortium are linked in part to the selection for non-PAO flanking microorganisms with truncated denitrification pathways. © 2019
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/121894
Appears in Collections:气候变化事实与影响

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作者单位: Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208, United States; College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China; Shenzhen Key Laboratory of Environmental Chemistry and Ecological Remediation, Shenzhen University, Shenzhen, 518060, China; Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong; Master of Science in Biotechnology Program, McCormick School of Engineering, Northwestern University, Evanston, IL 60208, United States; Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Champaign, IL 61801, United States

Recommended Citation:
Gao H.,Mao Y.,Zhao X.,et al. Genome-centric metagenomics resolves microbial diversity and prevalent truncated denitrification pathways in a denitrifying PAO-enriched bioprocess[J]. Water Research,2019-01-01,155
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