DOI: 10.1016/j.watres.2019.01.028
Scopus记录号: 2-s2.0-85061080061
论文题名: A cryogel-based bioreactor for water treatment applications
作者: Berillo D.A. ; Caplin J.L. ; Cundy A.B. ; Savina I.N.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 324
结束页码: 334
语种: 英语
英文关键词: Bacteria immobilisation
; Bioremediation
; Chlorophenols
; Cresol
; Phenol
Scopus关键词: Bacteria
; Biodegradation
; Biomechanics
; Bioreactors
; Bioremediation
; Biotechnology
; Composite materials
; Crosslinking
; Fluidized beds
; Mechanical properties
; Phenols
; Pore size
; Chlorophenols
; Cresol
; Enzymatic activities
; Immobilisation
; Laser scanning confocal microscopy
; One-step preparation methods
; Phenol and m-cresol
; Pseudomonas mendocina
; Biological water treatment
; 2 chlorophenol
; 4 chlorophenol
; carbon
; meta cresol
; phenol
; phenol derivative
; water
; bacterium
; biodegradation
; bioreactor
; bioremediation
; gel
; immobilization
; methodology
; phenolic compound
; water treatment
; Acinetobacter
; Acinetobacter radioresistens
; Article
; biodegradation
; bioremediation
; cell density
; confocal laser scanning microscopy
; controlled study
; cross linking
; cryogel
; enzyme activity
; freezing
; nonhuman
; pore size
; priority journal
; Pseudomonas mendocina
; Rhodococcus
; Rhodococcus koreensis
; scanning electron microscopy
; water treatment
; Acinetobacter radioresistens
; Pseudomonas mendocina
; Rhodococcus koreensis
英文摘要: The aim of this study was to develop and test a non-diffusion limited, high cell density bioreactor for biodegradation of various phenol derivatives. The bioreactor was obtained using a straightforward one-step preparation method using cryostructuration and direct cross-linking of bacteria into a 3D structured (sponge-like) macroporous cryogel composite material consisting of 11.6% (by mass) cells and 1.2–1.7% polymer, with approximately 87% water (in the material pores). The macroporous cryogel composite material, composed of live bacteria, has pore sizes in the range of 20–150 μm (confirmed by SEM and Laser Scanning Confocal Microscopy). The enzymatic activity of bacteria within the cryogel structure and the effect of freezing on the viability of the cross-linked cells was estimated by MTT assay. Cryogels based on Pseudomonas mendocina, Rhodococcus koreensis and Acinetobacter radioresistens were exploited for the effective bioremediation of phenol and m-cresol, and to a lesser extent 2-chlorophenol and 4-chlorophenol, utilising these phenolic contaminants in water as their only source of carbon. For evaluation of treatment scalability the bioreactors were prepared in plastic “Kaldnes” carriers to improve their mechanical properties and allow application in batch or fluidised bed water treatment modes. © 2019 The Author(s) Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/121971
Appears in Collections: 气候变化事实与影响
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作者单位: School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, United Kingdom; School of Environment and Technology, University of Brighton, Brighton, United Kingdom; School of Ocean and Earth Science, University of Southampton, Southampton, United Kingdom
Recommended Citation:
Berillo D.A.,Caplin J.L.,Cundy A.B.,et al. A cryogel-based bioreactor for water treatment applications[J]. Water Research,2019-01-01