DOI: 10.1016/j.watres.2019.01.009
Scopus记录号: 2-s2.0-85060988916
论文题名: Monitoring Cr toxicity and remediation processes - combining a whole-cell bioreporter and Cr isotope techniques
作者: Zhang Q. ; Song Y. ; Amor K. ; Huang W.E. ; Porcelli D. ; Thompson I.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 295
结束页码: 303
语种: 英语
英文关键词: Bacterial reduction
; Chromium
; Isotopes
; Whole-cell bioreporters
Scopus关键词: Bacteria
; Bioremediation
; Biotechnology
; Cells
; Chromium
; Cost effectiveness
; Cytology
; Detoxification
; Isotopes
; Toxicity
; Bacterial reduction
; Cost-effective means
; Fractionation factors
; Initial concentration
; Low concentrations
; Pseudomonas fluorescens
; Reduction mechanisms
; Whole cell
; Chromium compounds
; chromium
; bacterium
; bioluminescence
; bioreactor
; bioremediation
; chromium
; concentration (composition)
; detoxification
; genotoxicity
; isotopic analysis
; isotopic fractionation
; reduction
; Acinetobacter baylyi
; Article
; bioluminescence
; bioremediation
; cell fractionation
; DNA damage
; genotoxicity
; isotope tracing
; mathematical computing
; nonhuman
; priority journal
; Pseudomonas fluorescens
; whole cell
; Acinetobacter baylyi
; Bacteria (microorganisms)
; Pseudomonas fluorescens
英文摘要: Bioremediation is a sustainable and cost-effective means of contaminant detoxification. Although Cr(VI) is toxic at high concentrations, various microbes can utilise it as an electron accepter in the bioremediation process, and reduce it to the less toxic form Cr(III). During remediation, it is important to monitor the level of toxicity and effectiveness of Cr(VI) reduction in order to optimize the conditions. This study employed a whole-cell bioreporter Acinetobacter baylyi ADPWH-recA to access the degree of toxicity of different species of Cr over a range of initial concentrations. It also investigated whether Cr isotope fractionation factors were impacted by different levels of Cr toxicity (related to its concentration) and Cr(VI) reduction rates by Cr resistant bacteria Pseudomonas fluorescens LB 300. The results show that, of both Cr 2 O 7 2− and CrO 4 2− , the whole-cell bioreporter was efficient in indicating the level of genotoxicity of Cr(VI) at low concentrations and cytotoxicity at high concentrations via variations of bioluminescence. High concentrations (> 100 mg/L) of Cr(III) could also strongly induce the luminescence in the bioreporter, indicating DNA damage at such abundance. Pseudomonas fluorescens LB 300 was found to be effective in reducing Cr(VI) even when the concentration was high (40 mg/L); however, complete Cr(VI) reduction was only observed at low concentrations (< 5 mg/L), since the toxicity of high concentrations of Cr(VI) impacted the effectiveness of reduction by the bacteria. During reduction, the C53r/C52r ratio of remaining Cr(VI) increased from its initial value, and the calculated fractionation factor by bacterial Cr(VI) reduction (ε) was −3.1±0.3‰. The fractionation factor was independent of the initial Cr(VI) concentration. Therefore, a single Cr isotope fractionation factor can be effectively applied in indicating the extent of bioremediation processing of Cr(VI) over a wide range of concentrations. This significantly simplified monitoring of Cr(VI) depletion in bioremediation, since variations of ε normally indicate a change in the reduction mechanism and therefore would complicate the elucidation of processes driving the remediation. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/121975
Appears in Collections: 气候变化事实与影响
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作者单位: Department of Earth Sciences, University of Oxford, South Parks Road, OX1 3AN, Oxford, United Kingdom; Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, Oxford, United Kingdom
Recommended Citation:
Zhang Q.,Song Y.,Amor K.,et al. Monitoring Cr toxicity and remediation processes - combining a whole-cell bioreporter and Cr isotope techniques[J]. Water Research,2019-01-01