DOI: 10.1016/j.watres.2019.01.002
Scopus记录号: 2-s2.0-85060176324
论文题名: Diagnosing water treatment critical control points for cyanobacterial removal: Exploring benefits of combined microscopy, next-generation sequencing, and cell integrity methods
作者: Zamyadi A. ; Romanis C. ; Mills T. ; Neilan B. ; Choo F. ; Coral L.A. ; Gale D. ; Newcombe G. ; Crosbie N. ; Stuetz R. ; Henderson R.K.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 96
结束页码: 105
语种: 英语
英文关键词: Cell integrity
; Cyanobacteria
; Harmful metabolites
; Microscopic taxonomy
; Next-generation sequencing
; Water treatment
Scopus关键词: Clarification
; Climate change
; Cost effectiveness
; Cytology
; Metabolites
; Ozone
; Ozone water treatment
; Ozonization
; Potable water
; Potash
; Surface waters
; Taxonomies
; Wastewater reclamation
; Water conservation
; Water quality
; Water treatment
; Cell integrity
; Combined microscopies
; Critical control points
; Cyanobacteria
; Cyanobacterial species
; Next-generation sequencing
; Potassium permanganate
; Taxonomy identification
; Cells
; ozone
; permanganate potassium
; RNA 16S
; water
; algal bloom
; cell
; climate change
; cyanobacterium
; drinking water
; metabolite
; pollutant removal
; taxonomy
; water quality
; water treatment
; Actinomycetales
; algal bloom
; Article
; bacterial cell
; cell count
; cell damage
; cell fate
; cell structure
; cell viability
; Chlorophyceae
; cyanobacterium
; Cylindrospermopsis
; Enterobacteriales
; gene sequence
; green alga
; limit of detection
; microbiome
; Microcystis
; microscopy
; next generation sequencing
; nonhuman
; ozonation
; priority journal
; sedimentation
; supernatant
; water quality
; water sampling
; water supply
; water treatment
; Cyanobacteria
英文摘要: A wide range of cyanobacterial species and their harmful metabolites are increasingly detected in water bodies worldwide, exacerbated by climate change and human activities. The resulting bloom conditions represent significant challenges to production of safe drinking water and cost effective water reuse, therefore their removal is a priority to ensure public safety. While current microscopic taxonomy identification methods provide valuable information about cell numbers during treatment, these methods are incapable of providing information about the fate of cells during treatment. The objectives of this study were to (1) identify the critical control points for breakthrough and accumulation of cells by investigating the fate of cells during treatment processes using a combination of taxonomy, cell integrity and next-generation sequencing (NGS), and (2) assess the impact of pre-treatment processes on breakthrough prevention at critical control points, and the benefits of cell integrity and NGS analysis for improved management purposes. This paper presents the results of an unprecedented cyanobacterial monitoring program conducted in four full scale water treatment plants located in three different climate zones. Cyanobacterial cell integrity and accumulation during operation process were assessed for the first time using next generation of gene sequencing methods. NGS analysis led to detection of cyanobacterial and melainabacteria orders in water samples that were not identified by microscopy. 80 ± 5% of cells were completely lysed post pre-oxidation (for both ozone and potassium permanganate). However unlike pre-ozonation, the remaining cells were undamaged cells with the potential to accumulate and grow within the plants post-KMnO4 treatment, particularly in clarifier sludge. To effectively monitor water quality, this study presents a synergistic approach coupling new and traditional analytical methods and demonstrates the importance of identifying critical points for managing accumulation of cyanobacteria within plants. © 2019 Elsevier Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122010
Appears in Collections: 气候变化事实与影响
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作者单位: Water Research Centre, School of Civil and Environmental Engineering, University of New South Wales (UNSW), Sydney, Australia; BioMASS Lab, School of Chemical Engineering, UNSW, Sydney, Australia; BGA Innovation Hub, and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montreal, Canada; School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia; Chemistry and Biology Department, Universidade Tecnológica Federal do Paraná, Curitiba, Brazil; Seqwater, Ipswich, Australia; South Australian Water Corporation (SA Water), Adelaide, Australia; Melbourne Water, Melbourne, Australia
Recommended Citation:
Zamyadi A.,Romanis C.,Mills T.,et al. Diagnosing water treatment critical control points for cyanobacterial removal: Exploring benefits of combined microscopy, next-generation sequencing, and cell integrity methods[J]. Water Research,2019-01-01