DOI: 10.1016/j.watres.2018.12.069
Scopus记录号: 2-s2.0-85060154253
论文题名: New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes
作者: Ma L. ; Li B. ; Zhang T.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 191
结束页码: 201
语种: 英语
英文关键词: Antibiotic resistome
; Drinking water
; Metagenomic sequencing
; Pathogenic host
; Public health
Scopus关键词: Antibiotics
; Bacteria
; Genes
; Health risks
; Microfiltration
; Public health
; Water filtration
; Water supply
; Aminoglycoside resistance
; Antibiotic resistance genes
; Drinking water samples
; Metagenomic assemblies
; Metagenomic sequencing
; Mycobacterium gordonae
; Pathogenic host
; Pseudomonas aeruginosa
; Potable water
; aminoglycoside antibiotic agent
; bacitracin
; drinking water
; antibiotic resistance
; bacterium
; disinfection
; drinking water
; genomics
; pathogen
; public health
; water management
; antibiotic resistance
; Article
; bacterium identification
; China
; disinfection
; Hong Kong
; metagenomics
; Mycobacterium gordonae
; nonhuman
; priority journal
; Pseudomonas aeruginosa
; Pseudomonas alcaligenes
; Singapore
; water management
; water sampling
; China
; Hong Kong
; Singapore [Singapore (NTN)]
; Singapore [Southeast Asia]
; Bacteria (microorganisms)
; Mycobacterium gordonae
; Pseudomonas aeruginosa
; Pseudomonas alcaligenes
英文摘要: The proliferation of antibiotic resistance genes (ARGs) in drinking water and their potential horizontal transfer to pathogenic microbes may cause failure of antibiotics. However, antimicrobial resistome monitoring in drinking water is not currently routine. The bacterial hosts of ARGs, especially small-sized microbes in drinking water, may not be effectively removed by membrane filtration disinfection and thus pose threats to human health. In the present study, using metagenomic based approach, we investigated antibiotic resistome of small-sized microbes (0.2–0.45 μm) in 20 household drinking water samples from 12 cities in Mainland China, Hong Kong and Singapore. A total of 265 ARG subtypes belonging to 17 ARG types were detected at abundances ranging from 4.0 × 10 −2 to 1.0 × 10 0 copies/cell. Multidrug, bacitracin and aminoglycoside resistance genes are dominant, and 43 ARG subtypes were specifically carried by small-size microbes. Metagenomic assembly strategy revealed fragments of three opportunistic pathogen, i.e. Pseudomonas alcaligenes, Pseudomonas aeruginosa and Mycobacterium gordonae, carried mexW, aph(3′)-I and aac(2′)-I, respectively. Drinking water samples were classified into three groups based on the presence of ARG, pathogen and ARG-carrying pathogen. These new insights into the antibiotic resistome of small microbes in drinking water over a broad scale indicate the need for more comprehensive ARGs monitoring and surveillance of drinking water supplies. These findings, together with the perspectives and strategies proposed in this study, could support initiatives to improve drinking water safety. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122013
Appears in Collections: 气候变化事实与影响
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作者单位: Environmental Biotechnology Laboratory, The University of Hong Kong, Hong Kong; Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, China; Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University, China; School of Public Health, The University of Hong Kong, Hong Kong; International Center for Antibiotic Resistance in the Environment, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, China
Recommended Citation:
Ma L.,Li B.,Zhang T.. New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes[J]. Water Research,2019-01-01