DOI: 10.1016/j.watres.2018.12.034
Scopus记录号: 2-s2.0-85059458240
论文题名: Deciphering of microbial community and antibiotic resistance genes in activated sludge reactors under high selective pressure of different antibiotics
作者: Zhao R. ; Feng J. ; Liu J. ; Fu W. ; Li X. ; Li B.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 388
结束页码: 402
语种: 英语
英文关键词: Activated sludge
; Antibiotic resistance genes
; Antibiotics
; Metagenomic analysis
; Microbial community
; Selective pressure
Scopus关键词: Activated sludge process
; Batch reactors
; Genes
; Microorganisms
; RNA
; Wastewater treatment
; Activated sludge
; Antibiotic resistance genes
; Metagenomic analysis
; Microbial communities
; Selective pressure
; Antibiotics
; aminoglycoside
; antibiotic agent
; bacitracin
; chloramphenicol
; tetracycline
; trimethoprim
; abundance estimation
; activated sludge
; antibiotic resistance
; antibiotics
; bioreactor
; bioremediation
; gene
; genetic analysis
; genome
; microbial community
; species diversity
; activated sludge
; amplicon
; antibiotic resistance
; antibiotic resistance gene
; antibiotic therapy
; Aridibacter
; Arthrobacter
; Article
; Conexibacter
; drug manufacture
; gene
; gene sequence
; Geothrix
; metagenomics
; microbial community
; municipal wastewater
; Nitrosomonas
; nonhuman
; phylum
; pressure
; priority journal
; Proteobacteria
; Rudaea
; Terrimonas
; Thauera
; Thiobacillus
; Arthrobacter
; Conexibacter
; Geothrix
; Thauera
英文摘要: Currently, the effects of high antibiotic concentrations on the performance of microbiota and antibiotic resistance genes (ARGs) in activated sludge (AS) process are not well characterized. Lab-scale batch reactors were performed to evaluate the dynamics of microbial community and ARGs in response to six antibiotics at different concentrations using high-throughput sequencing-based 16S rRNA gene and metagenomic analyses. The presence of antibiotics remarkably decreased the microbial diversity, caused a great change of the microbiota structure, and exerted a selective pressure on the enrichment of potential antibiotic resistant bacteria (ARB), such as Arthrobacter, Thauera, Geothrix, Rudaea, Aridibacter, Conexibacter, Terrimonas, etc. High antibiotic selective pressures increased ARG abundance but simultaneously reduced ARG number. In total, 491 ARG subtypes belonging to 20 ARG types were detected and kanamycin treatment showed the highest ARG abundances. A core set of 54 ARG subtypes that accounted for 66.7%–99.6% of the total ARG abundances were shared by all samples. The increase of the abundances of both corresponding and non-corresponding ARGs under a specific antibiotic treatment revealed the collateral effects of antibiotic selective pressure. Microbial community may play an important role in the composition of ARGs. Network analysis indicated that both internal-type and external-type of ARGs exhibited higher non-random co-occurrence incidences and 18 genera were speculated as the possible hosts for multiple ARGs. This study deciphered the profiles and relationships between microbial community and ARGs in AS process treating wastewater with high antibiotic concentrations and could provide helpful guidance for controlling the development and dissemination of ARB and ARGs. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122043
Appears in Collections: 气候变化事实与影响
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作者单位: Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China; School of Environment, Tsinghua University, Beijing, 100084, China
Recommended Citation:
Zhao R.,Feng J.,Liu J.,et al. Deciphering of microbial community and antibiotic resistance genes in activated sludge reactors under high selective pressure of different antibiotics[J]. Water Research,2019-01-01