DOI: 10.1016/j.watres.2018.12.003
Scopus记录号: 2-s2.0-85058776736
论文题名: Impact of blending for direct potable reuse on premise plumbing microbial ecology and regrowth of opportunistic pathogens and antibiotic resistant bacteria
作者: Garner E. ; Inyang M. ; Garvey E. ; Parks J. ; Glover C. ; Grimaldi A. ; Dickenson E. ; Sutherland J. ; Salveson A. ; Edwards M.A. ; Pruden A.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 75
结束页码: 86
语种: 英语
英文关键词: Antibiotic resistance genes
; Biological stability
; Direct potable reuse
; Opportunistic pathogens
; Premise plumbing
Scopus关键词: Antibiotics
; Bacteria
; Biofilms
; Blending
; Ecology
; Gene transfer
; Genes
; Plumbing
; Polyvinyl chlorides
; RNA
; Water conservation
; Water quality
; Antibiotic resistance genes
; Antibiotic-resistant bacteria
; Biological stability
; Heterotrophic plate counts
; Microbial community composition
; Next-generation sequencing
; Potable reuse
; Premise plumbings
; Potable water
; biodegradable dissolved organic carbon
; drinking water
; ground water
; organic carbon
; RNA 16S
; surface water
; unclassified drug
; antibiotic resistance
; bacterium
; bioactivity
; biotechnology
; drinking water
; gene
; microbial ecology
; pathogen
; recycling
; regrowth
; water quality
; water treatment
; amplicon
; antibiotic resistance
; Article
; bacterial virulence
; biofilm
; biological activity
; biological activity reaction test
; direct potable reuse
; ecology
; horizontal gene transfer
; incubation time
; Legionella
; marker gene
; microbial community
; multidrug resistance
; Mycobacterium
; next generation sequencing
; priority journal
; Pseudomonas aeruginosa
; waste water management
; water management
; water quality
; water supply
; water treatment
; Legionella
; Mycobacterium
; Pseudomonas aeruginosa
英文摘要: Little is known about how introducing recycled water intended for direct potable reuse (DPR) into distribution systems and premise plumbing will affect water quality at the point of use, particularly with respect to effects on microbial communities and regrowth. The examination of potential growth of opportunistic pathogens (OPs) and spread of antibiotic resistance genes (ARGs), each representing serious and growing public health concerns, by introducing DPR water has not previously been evaluated. In this study, the impact of blending purified DPR water with traditional drinking water sources was investigated with respect to treatment techniques, blending location, and blending ratio. Water from four U.S. utility partners was treated in bench- and pilot-scale treatment trains to simulate DPR with blending. Water was incubated in simulated premise plumbing rigs made of PVC pipe containing brass coupons to measure regrowth of total bacteria (16S rRNA genes, heterotrophic plate count), OPs (Legionella spp., Mycobacterium spp., Pseudomonas aeruginosa), ARGs (qnrA, vanA), and an indicator of horizontal gene transfer and multi-drug resistance (intI1). The microbial community composition was profiled and the resistome (i.e., all ARGs present) was characterized in select samples using next generation sequencing. While regrowth of total bacteria (16S rRNA genes) from the start of the incubation through week eight consistently occurred across tested scenarios (Wilcoxon, p ≤ 0.0001), total bacteria were not more abundant in the water or biofilm of any DPR scenario than in the corresponding conventional potable condition (p ≥ 0.0748). Regrowth of OP marker genes, qnrA, vanA, and intI1 were not significantly greater in water or biofilm for any DPR blends treated with advanced oxidation compared to corresponding potable water (p ≥ 0.1047). This study of initial bacteria colonizing pipes after introduction of blended DPR water revealed little evidence (i.e., one target in one water type) of exacerbated regrowth of total bacteria, OPs, or ARGs in premise plumbing. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122071
Appears in Collections: 气候变化事实与影响
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作者单位: Via Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA 24061, United States; Southern Nevada Water Authority, Henderson, NV 89015, United States; Carollo Engineers, Inc, Walnut Creek, CA 94598, United States; Massachusetts Water Resources Authority, Southborough, MA 01772, United States
Recommended Citation:
Garner E.,Inyang M.,Garvey E.,et al. Impact of blending for direct potable reuse on premise plumbing microbial ecology and regrowth of opportunistic pathogens and antibiotic resistant bacteria[J]. Water Research,2019-01-01