globalchange  > 气候变化事实与影响
DOI: 10.1016/j.watres.2018.11.039
Scopus ID: 2-s2.0-85057845957
Title:
Integrated zebrafish-based tests as an investigation strategy for water quality assessment
Author: Shao Y.; Xiao H.; Di Paolo C.; Deutschmann B.; Brack W.; Hollert H.; Seiler T.B.
Source Publication: Water Research
ISSN: 431354
Publishing Year: 2019
pages begin: 252
pages end: 260
Language: 英语
Keyword: Large throughput screening ; Tiered bioassays ; Toxicity-based mixture elucidation ; Zebrafish
Scopus Keyword: Bioassay ; Environmental regulations ; Gene expression ; Health risks ; Mixtures ; Risk assessment ; Throughput ; Toxicity ; Water conservation ; Water quality ; Aryl hydrocarbon receptor ; Bioanalytical techniques ; Embryonic development ; Ethoxyresorufin-O-deethylase ; European Water Framework Directive ; Throughput screening ; Water quality assessments ; Zebrafish ; Water pollution ; aromatic hydrocarbon receptor ; cytochrome P450 1A ; endocrine disruptor ; RNA ; surface water ; bioassay ; cell component ; cyprinid ; embryonic development ; European Union ; gene expression ; genotoxicity ; health risk ; pollution exposure ; protein ; risk assessment ; RNA ; testing method ; toxicity ; water quality ; animal cell ; Article ; bioassay ; cell damage ; controlled study ; embryo ; embryo development ; enzyme activity ; gene expression ; gene expression assay ; genotoxicity ; high performance liquid chromatography ; in vitro study ; in vivo study ; larval stage ; micronucleus ; micronucleus test ; nonhuman ; priority journal ; quality control ; risk assessment ; teratogenicity ; water quality ; water sampling ; zebra fish ; Danube River ; Europe ; Danio rerio
English Abstract: Water pollution risks to human health and the environment are emerging as serious concerns in the European Union and worldwide. With the aim to achieve good ecological and chemical status of all European water bodies, the “European Water Framework Directive” (WFD) was enacted. With the framework, bioanalytical techniques have been recognized as an important aspect. However, there are limitations to the application of bioassays directly for water quality assessment. Such approaches often fail to identify pollutants of concern, since the defined priority and monitored pollutants often fail to explain the observed toxicity. In this study, we integrated an effect-based risk assessment with a zebrafish-based investigation strategy to evaluate water sample extracts and fractions collected from the Danube. Four tiered bioassays were implemented, namely RNA-level gene expression assay, protein-level ethoxyresorufin-O-deethylase (EROD) assay, cell-level micronucleus assay and organism-level fish embryo test (FET). The results show that teratogenicity and lethality during embryonic development might be induced by molecular or cellular damages mediated by the aryl hydrocarbon receptor (AhR) -mediated activity, estrogenic activity and genotoxic activity. With the combination of high-throughput fractionation, this effect-based strategy elucidated the major responsible mixtures of each specific toxic response. In particularly, the most toxic mixture in faction F4, covering a log Kow range from 2.83 to 3.42, was composed by 12 chemicals, which were then evaluated as a designed mixture. Our study applied tiered bioassays with zebrafish to avoid interspecies differences and highlights effect-based approaches to address toxic mixtures in water samples. This strategy can be applied for large throughput screenings to support the main toxic compounds identification in water quality assessment. © 2018 Elsevier Ltd
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被引频次[WOS]:11   [查看WOS记录]     [查看WOS中相关记录]
Document Type: 期刊论文
Identifier: http://119.78.100.158/handle/2HF3EXSE/122086
Appears in Collections:气候变化事实与影响

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Affiliation: Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), ABBt – Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, Aachen, 52074, Germany; UFZ – Helmholtz Centre for Environmental Research GmbH, Department of Cell Toxicology, Permoserstraße 15, Leipzig, 04318, Germany; UFZ – Helmholtz Centre for Environmental Research GmbH, Department for Effect-Directed Analysis, Permoserstraße 15, Leipzig, 04318, Germany; College of Resources and Environmental Science, Chongqing University, 174 Shazheng Road Shapingba, Chongqing, 400044, China; College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai, 200092, China; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 200023, China

Recommended Citation:
Shao Y.,Xiao H.,Di Paolo C.,et al. Integrated zebrafish-based tests as an investigation strategy for water quality assessment[J]. Water Research,2019-01-01
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