DOI: 10.1016/j.watres.2018.11.012
Scopus记录号: 2-s2.0-85056645352
论文题名: Genome-resolved metagenomic analysis reveals roles of microbial community members in full-scale seawater reverse osmosis plant
作者: Rehman Z.U. ; Ali M. ; Iftikhar H. ; Leiknes T.O.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 263
结束页码: 271
语种: 英语
英文关键词: Membrane biofouling
; Membrane-based water treatment
; Metagenomics
; Reverse osmosis
; Seawater desalination
Scopus关键词: Biofilms
; Biofouling
; Desalination
; Genes
; Membranes
; Microorganisms
; Nitrates
; Quenching
; Seawater
; Water treatment
; Membrane biofouling
; Membrane-based
; Metagenomic analysis
; Metagenomics
; Microbial communities
; Seawater desalination
; Seawater reverse osmosis plants
; Selective pressure
; Reverse osmosis
; nitrate
; sea water
; antimicrobial activity
; bacterium
; biofilm
; biofouling
; cell component
; desalination
; genome
; genomics
; membrane
; microbial activity
; microbial community
; reverse fault
; reverse osmosis
; seawater
; water industry
; water treatment
; amino acid sequence
; Article
; Bacteroidetes
; Betaproteobacteria
; biofilm
; biofouling
; bootstrapping
; controlled study
; Deltaproteobacteria
; DNA extraction
; gene sequence
; metagenomics
; microbial community
; microbial diversity
; nitrogen cycling
; nonhuman
; phylogenetic tree
; Planctomycetes
; priority journal
; Proteobacteria
; quorum sensing
; reverse osmosis
; surface property
; water treatment
; Bacteria (microorganisms)
; Bacteroidetes
; Planctomycetes
; Proteobacteria
英文摘要: Biofouling of Reverse Osmosis (RO) membrane is a significant issue for the water treatment industry. In this study, we apply the metagenomic shot-gun sequencing technology to characterise the composition and functional potential of the microbial community in a full-scale RO plant, at different stages of seawater treatment. We find Proteobacteria, Bacteroidetes and Planctomycetes to be the most abundant bacterial phyla. The genetic potential of the RO membrane microbial community shows the enrichment of genes involved in biofilm formation, representing the selective pressure of the biofilm formation process. We recover 31 metagenome-assembled genomes (MAGs) from intake (raw seawater), fouled RO membranes (leading and middle RO module) and brine reject water. A total of 25 MAGs are recovered from the biofilm samples (leading and middle RO modules), with 9 of them (36%) belonging to Planctomycetes. We investigate all 25 MAGs for genes (pili, flagella, quorum sensing, quorum quenching and nitrate reduction) that play an important role in biofilm formation and sustenance of cells. We show that Planctomycetes contain genes for the formation of flagella and pili, and the reduction of nitrate. Although genes for quorum sensing are not detected, quorum quenching genes are identified in the biofilm MAGs. Our results show that Planctomycetes, along with other microbes, play an important role in the formation and sustenance of biofilms on seawater RO membranes. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122154
Appears in Collections: 气候变化事实与影响
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作者单位: Water Desalination and Reuse Center (WDRC), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia
Recommended Citation:
Rehman Z.U.,Ali M.,Iftikhar H.,et al. Genome-resolved metagenomic analysis reveals roles of microbial community members in full-scale seawater reverse osmosis plant[J]. Water Research,2019-01-01