DOI: 10.1016/j.watres.2018.10.072
Scopus记录号: 2-s2.0-85055915584
论文题名: Reactivation and pilot-scale application of long-term storage denitrification biofilm based on flow cytometry
作者: Wang S. ; Qian K. ; Zhu Y. ; Yi X. ; Zhang G. ; Du G. ; Tay J.-H. ; Li J.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 368
结束页码: 377
语种: 英语
英文关键词: Denitrification biofilm
; Flow cytometry
; Long-term preservation
; Microbial community structure
; TB-EPS
Scopus关键词: Biofilms
; Cell adhesion
; Cells
; Flow cytometry
; Denitrification capacity
; Extra-cellular polymeric substances
; Flow cytometry analysis
; Long-term preservation
; Microbial characteristics
; Microbial community structures
; Pilot scale application
; Positive correlations
; Denitrification
; nitrate
; polymer
; adhesion
; bacterium
; biofilm
; cell component
; community structure
; denitrification
; exopolymer
; flow cytometry
; microbial activity
; microbial community
; microbial ecology
; protein
; reactivation
; reduction
; Acidovorax
; Aquabacterium
; Article
; bacterium
; biofilm
; cell viability
; correlation analysis
; Dechloromonas
; denitrification
; Flavobacteriaceae
; flow cytometry
; microbial adhesion
; nonhuman
; Parcubacteria
; preservation and storage
; prevalence
; priority journal
; protein content
; Pseudomonas
; Acidovorax
; Aquabacterium
; Dechloromonas
; Pseudomonas
; unclassified Flavobacteriaceae
英文摘要: The work provides a method on the basis of flow cytometry to evaluate the performance of denitrification biofilm during the preservation, reactivation and pilot-scale operation process. The viable cell ratio of denitrification biofilm significantly reduced and further led to the decrease of denitrification capacity after long-term preservation for 5 months. Protein component in tightly bound extracellular polymeric substances (TB-EPS) could serve to enhance microbial adhesion and promote denitrification biofilm formation. With the significant correlation of viable cell ratio and microbial characteristics, 4 °C was more appropriate for preserving denitrification biofilm and conducive to maintain the relatively high denitrification capacity. A maximum denitrification rate of 5.80 gNO 3 - -N/m 2 ·d was obtained in pilot-scale anoxic-oxic (AO) process and Dechloromonas became greater prevalence in denitrification suspended carriers. Furthermore, the enrichment of Pseudomonas, Parcubacteria, Acidovorax, Aquabacterium and Unclassified_Flavobacteriaceae enhanced biofilm formation and nutrient conservation. The significantly positive correlation between viable cell ratio and the ratio of nitrate reduction to COD consumption was discovered, and the indices of Chao, ACE, Shannon and Simpson of denitrification biofilm were positively correlated with viable cell ratio, meaning that flow cytometry analysis was reasonable and suitable to evaluate the performances of denitrification biofilm. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122192
Appears in Collections: 气候变化事实与影响
There are no files associated with this item.
作者单位: Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi, 214122, China; Jiangsu College of Water Treatment Technology and Material Collaborative Innovation Center, Suzhou, 215009, China; Department of Civil Engineering, Schulich School of Engineering, University of Calgary, Calgary, T2N 1N4, Canada; School of Environmental Science and Engineering, Hainan University, Haikou, 570028, China; Ministry Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China
Recommended Citation:
Wang S.,Qian K.,Zhu Y.,et al. Reactivation and pilot-scale application of long-term storage denitrification biofilm based on flow cytometry[J]. Water Research,2019-01-01