Scopus记录号: 2-s2.0-85059497776
论文题名: Windows into microbial seascapes: Advances in nanoscale imaging and application to marine sciences
作者: Taylor G.T.
刊名: Annual Review of Marine Science
ISSN: 19411405
出版年: 2019
卷: 11 起始页码: 465
结束页码: 490
语种: 英语
英文关键词: atomic force microscopy
; confocal Raman microspectroscopy
; FISH
; fluorescence in situ hybridization
; intracellular mapping
; nanoscale secondary ion mass spectrometry
; nanoSIMS
; single-cell analysis
Scopus关键词: Atomic force microscopy
; Ecology
; Fish
; Fluorescence microscopy
; Geochemistry
; Marine applications
; Microorganisms
; Secondary emission
; Secondary ion mass spectrometry
; Confocal Raman microspectroscopy
; Fluorescence in situ hybridization
; Nano scale
; NanoSIMS
; Singlecell analysis
; Nanotechnology
; atomic force microscopy
; cell component
; detection method
; ecophysiology
; genetic analysis
; imaging method
; mapping method
; marine environment
; mass spectrometry
; microbiology
; microorganism
; pelagic environment
; phenotype
英文摘要: Geochemical cycles of all nonconservative elements are mediated by microorganisms over nanometer spatial scales. The pelagic seascape is known to possess microstructure imposed by heterogeneous distributions of particles, polymeric gels, biologically important chemicals, and microbes. While indispensable, most traditional oceanographic observational approaches overlook this heterogeneity and ignore subtleties, such as activity hot spots, symbioses, niche partitioning, and intrapopulation phenotypic variations, that can provide a deeper mechanistic understanding of planktonic ecosystem function. As part of the movement toward cultivation-independent tools in microbial oceanography, techniques to examine the ecophysiology of individual populations and their role in chemical transformations at spatial scales relevant to microorganisms have been developed. This review presents technologies that enable geochemical and microbiological interrogations at spatial scales ranging from 0.02 to a few hundred micrometers, particularly focusing on atomic force microscopy, nanoscale secondary ion mass spectrometry, and confocal Raman microspectroscopy and introducing promising approaches for future applications in marine sciences. © Copyright 2019 by Annual Reviews. All rights reserved. 资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122792
Appears in Collections: 气候变化事实与影响
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作者单位: School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794, United States
Recommended Citation:
Taylor G.T.. Windows into microbial seascapes: Advances in nanoscale imaging and application to marine sciences[J]. Annual Review of Marine Science,2019-01-01,11