globalchange  > 气候减缓与适应
DOI: 10.1016/j.marenvres.2018.10.017
WOS记录号: WOS:000458595600002
论文题名:
The transcriptome of the marine calanoid copepod Temora longicornis under heat stress and recovery
作者: Semmouri, Ilias1; Asselman, Jana1; Van Nieuwerburgh, Filip2; Deforce, Dieter2; Janssen, Colin R.1; De Schamphelaere, Karel A. C.1
通讯作者: Semmouri, Ilias
刊名: MARINE ENVIRONMENTAL RESEARCH
ISSN: 0141-1136
EISSN: 1879-0291
出版年: 2019
卷: 143, 页码:10-23
语种: 英语
英文关键词: North sea ; BPNS ; Global warming ; Global change ; Climate change ; Temperature ; Gene expression ; Crustacea ; Arthropoda ; Zooplankton
WOS关键词: DIFFERENTIALLY EXPRESSED GENES ; APOLIPOPROTEIN-D ; PELAGIC COPEPODS ; SHOCK PROTEINS ; CLIMATE-CHANGE ; BELGIAN PART ; TIME-SERIES ; ZOOPLANKTON ; TEMPERATURE ; GENOME
WOS学科分类: Environmental Sciences ; Marine & Freshwater Biology ; Toxicology
WOS研究方向: Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
英文摘要:

Understanding the impacts of global change in zooplankton communities is crucial, as alterations in the zoo plankton communities can affect entire marine ecosystems. Despite the economic and ecological importance of the calanoid copepod Temora longicornis in the Belgian part of the North Sea, molecular data is still very limited for this species. Using HiSeq Illumina sequencing, we sequenced the whole transcriptome of T. longicornis, after being exposed to realistic temperatures of 14 and 17 degrees C. After both an acute (1 day) and a more sustained (5 days) thermal exposure to 17 degrees C, we investigated gene expression differences with animals exposed to 14 degrees C, which may be critical for the thermal acclimation and resilience of this copepod species. We also studied the possibility of a short term stress recovery of a heat shock. A total of 179,569 transcripts were yielded, of which 44,985 putative ORF transcripts were identified. These transcripts were subsequently annotated into roughly 22,000 genes based on known sequences using Gene Ontology (GO) and KEGG databases. Temora only showed a mild response to both the temperature and the duration of the exposure. We found that the expression of 27 transcripts varied significantly with an increase in temperature of 3 degrees C, of which eight transcripts were differentially expressed after acute exposure only. Gene set enrichment analysis revealed that, overall, T. longicornis was more impacted by a sustained thermal exposure, rather than an immediate (acute) exposure, with two times as many enriched GO terms in the sustained treatment. We also identified several general stress responses independent of exposure time, such as modified protein synthesis, energy mobilisation, cuticle and chaperone proteins. Finally, we highlighted candidate genes of a possible recovery from heat exposure, identifying similar terms as those enriched in the heat treatments, i.e. related to for example energy metabolism, cuticle genes and extracellular matrix. The data presented in this study provides the first transcriptome available for T. longicornis which can be used for future genomic studies.


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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/124976
Appears in Collections:气候减缓与适应

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作者单位: 1.Univ Ghent, Lab Environm Toxicol & Aquat Ecol, Fac Biosci Engn, B-9000 Ghent, Belgium
2.Univ Ghent, Lab Pharmaceut Biotechnol, Fac Pharmaceut Sci, B-9000 Ghent, Belgium

Recommended Citation:
Semmouri, Ilias,Asselman, Jana,Van Nieuwerburgh, Filip,et al. The transcriptome of the marine calanoid copepod Temora longicornis under heat stress and recovery[J]. MARINE ENVIRONMENTAL RESEARCH,2019-01-01,143:10-23
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