Sunflower and rapeseed are among the most important sources of vegetable oil for food and industry. The main components of vegetable oil are triglycerides (TAGs) (about 97%). Ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS) profiling of TAGs in sunflower and rapeseed has been performed and the TAG profiles obtained for these species have been compared. It has been identified that 34 TAGs are shared by sunflower and rapeseed. It was demonstrated that TAGs 52:2, 52:5, 52:6, 54:3; 54:4, 54:7, 56:3, 56:4, and 56:5 had the highest variability levels between sunflower and rapeseed with the higher presence in rapeseed. TAGs 50:2, 52:3, 52:4, 54:5, and 54:6 also showed high variability, but were the most abundant in sunflower. Moreover, the differences in TAG composition between the winter-type and spring-type rapeseed have been revealed, which may be associated with freezing tolerance. It was shown that winter-type rapeseed seeds contain TAGs with a lower degree of saturation, while in spring-type rapeseed highly saturated lipids are the most abundant. These findings may give new insights into the cold resistance mechanisms in plants the understanding of which is especially important in terms of global climate changes.
1.Skolkovo Inst Sci & Technol, Ctr Life Sci, Nobel St 3,Bldg 1, Moscow 121205, Russia 2.Russian Acad Sci, Inst Informat Transmiss Problems, Kharkevich Inst, Moscow 127051, Russia 3.Natl Res Univ, Fac Comp Sci, Higher Sch Econ, Moscow 119991, Russia 4.Russian Acad Sci, Inst Gen Genet, Gubkin St 3, Moscow 119991, Russia 5.Pustovoit All Russia Res Inst Oil Crops, Filatova St 17, Krasnodar 350038, Russia 6.Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Leninskie Gory 1,Bldg 40, Moscow 119234, Russia 7.All Russia Rice Res Inst, Belozerny 3, Krasnodar 350921, Russia
Recommended Citation:
Chernova, Alina,Gubaev, Rim,Mazin, Pavel,et al. UPLC-MS Triglyceride Profiling in Sunflower and Rapeseed Seeds[J]. BIOMOLECULES,2019-01-01,9(1)