globalchange  > 气候减缓与适应
DOI: 10.1007/s40333-018-0071-5
WOS记录号: WOS:000458965000009
论文题名:
Changes in soil microbial community response to precipitation events in a semi-arid steppe of the Xilin River Basin, China
作者: Zhang Hui1,2; Liu Wenjun2; Kang Xiaoming3; Cui Xiaoyong2; Wang Yanfen2; Zhao Haitao4; Qian Xiaoqing1,4; Hao Yanbin2
通讯作者: Qian Xiaoqing
刊名: JOURNAL OF ARID LAND
ISSN: 1674-6767
EISSN: 2194-7783
出版年: 2019
卷: 11, 期:1, 页码:97-110
语种: 英语
英文关键词: extreme precipitation event ; phospholipid fatty acid (PLFA) ; soil microbial community ; RT-qPCR ; soil bacteria ; soil fungi
WOS关键词: ALTERED PRECIPITATION ; INNER-MONGOLIA ; FUNGAL COMMUNITIES ; OXIDIZING BACTERIA ; EXTRACTION METHOD ; SEVERE DROUGHT ; CARBON ; NITROGEN ; RESISTANCE ; DIVERSITY
WOS学科分类: Environmental Sciences
WOS研究方向: Environmental Sciences & Ecology
英文摘要:

In the context of climate change, precipitation is predicted to become more intense at the global scale. Such change may alter soil microbial communities and the microbially mediated carbon and nitrogen dynamics. In this study, we experimentally repackaged precipitation patterns during the growing season (from June to September) of 2012 in a semi-arid temperate steppe of the Xilin River Basin in Inner Mongolia of China, based on the 60-year growing season precipitation data. Specifically, a total amount of 240 mm simulated precipitation was assigned to experimental plots by taking the following treatments: (1) P6 (6 extreme precipitation events, near the 1st percentile); (2) P10 (10 extreme precipitation events, near the 5(th) percentile); (3) P16 (16 moderate precipitation events, near the 50(th) percentile); and (4) P24 (24 events, 60-year average precipitation, near the 50(th) percentile). At the end of the growing season, we analyzed soil microbial community structure and biomass, bacterial abundance, fungal abundance and bacterial composition, by using phospholipid fatty acid (PLFA), real-time quantitative polymerase chain reaction (RT-qPCR) and 16S rRNA gene clone library methods. The extreme precipitation events did not change soil microbial community structure (represented by the ratio of PLFA concentration in fungi to PLFA concentration in bacteria, and the ratio of PLFA concentration in gram-positive bacterial biomass to PLFA concentration in gram-negative bacterial biomass). However, the extreme precipitation events significantly increased soil microbial activity (represented by soil microbial biomass nitrogen and soil bacterial 16S rRNA gene copy numbers). Soil fungal community showed no significant response to precipitation events. According to the redundancy analysis, both soil microbial biomass nitrogen and soil ammonium nitrogen (NH4-N) were found to be significant in shaping soil microbial community. Acidobacteria, Actinobacteria and Proteobacteria were the dominant phyla in soil bacterial composition, and responded differently to the extreme precipitation events. Based on the results, we concluded that the extreme precipitation events altered the overall soil microbial activity, but did not impact how the processes would occur, since soil microbial community structure remained unchanged.


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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/128496
Appears in Collections:气候减缓与适应

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作者单位: 1.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Jiangsu, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
3.Chinese Acad Forestry, Inst Wetland Res, Beijing 100091, Peoples R China
4.Yangzhou Univ, Coll Environm Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China

Recommended Citation:
Zhang Hui,Liu Wenjun,Kang Xiaoming,et al. Changes in soil microbial community response to precipitation events in a semi-arid steppe of the Xilin River Basin, China[J]. JOURNAL OF ARID LAND,2019-01-01,11(1):97-110
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