全球变化知识资源中心

Knowledge Resources Center for Global Change


Email:
Passwd
验证:
	换一张
Have you forgotten your password? Stay signed in Log In

globalchange > 气候减缓与适应

DOI:	10.1016/j.watres.2016.05.072
论文题名:	Real-time monitoring of beta-d-glucuronidase activity in sediment laden streams: A comparison of prototypes
作者:	Philipp Stadler^a; ^b; ; ¹; ; Gü; nter Blö; schl^a; ^c; ¹; Wolfgang Vogl^d; Juri Koschelnik^d; Markus Epp^d; Maximilian Lackner^d; Markus Oismü; ller^a; ¹; Monika Kumpan^e; ²; Lukas Nemeth^b; Peter Strauss^e; ²; Regina Sommer^f; ^g; ³; ⁴; Gabriela Ryzinska-Paier^h; Andreas H. Farnleitner^f; ^h; ³; Matthias Zessner^a; ^b; ¹
刊名:	water Research
ISSN:	0043-1354
出版年:	2016
卷:	Volume 101, 页码:Pages 252-261
语种:	英语
英文关键词:	Enzymatic activity ; Escherichia coli ; Faecal pollution ; Glucuronidase ; Online monitoring ; Water quality
英文摘要:	Detection of enzymatic activities has been proposed as a rapid surrogate for the culture-based microbiological pollution monitoring of water resources. This paper presents the results of tests on four fully automated prototype instruments for the on-site monitoring of beta-d-glucuronidase (GLUC) activity. The tests were performed on sediment-laden stream water in the Hydrological Open Air Laboratory (HOAL) during the period of March 2014 to March 2015. The dominant source of faecal pollution in the stream was swine manure applied to the fields within the catchment. The experiments indicated that instrument pairs with the same construction design yielded highly consistent results (R2=0.96 and R2=0.94), whereas the results between different designs were less consistent (R2=0.71). Correlations between the GLUC activity measured on-site and culture-based Escherichia coli analyses over the entire study period yielded R2=0.52 and R2=0.47 for the two designs, respectively. The correlations tended to be higher at the event scale. The GLUC activity was less correlated with suspended sediment concentrations than with E.coli, which is interpreted in terms of indicator applicability and the time since manure application. The study shows that this rapid assay can yield consistent results over a long period of on-site operation in technically challenging habitats. Although the use of GLUC activity as a proxy for culture-based assays could not be proven for the observed habitat, the study results suggest that this biochemical indicator has high potential for implementation in early warning systems.
URL:	http://www.sciencedirect.com/science/article/pii/S0043135416303980
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/12925
Appears in Collections:	气候减缓与适应气候变化与战略

Files in This Item:	Download All

File Name/ File Size	Content Type	Version	Access	License
1-s2.0-S0043135416303980-main.pdf（2134KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

^a TU Wien, Centre for Water Resource Systems, Karlsplatz 13, A-1040 Vienna, Austriaid^b TU Wien, Institute for Water Quality, Resources and Waste Management, Karlsplatz 13, A-1040 Vienna, Austriaid="aff3">^c TU Wien, Institute of Hydraulic Engineering and Water Resources Management, Karlsplatz 13/222, A-1040 Vienna, Austriaid="aff4">^d Vienna Water Monitoring, Dorfstrasse 17, A-2295 Zwerndorf, Austriaid="aff5">^e Federal Agency for Water Management, Institute for Land & Water Management Research, 3252 Petzenkirchen, Austriaid="aff6">^f Interuniversity Cooperation Centre for Water and Health, Gumpendorferstraße 1a, A-1060 Vienna, Austriaid="aff7">^g Medical University of Vienna, Institute for Hygiene and Applied Immunology, Water Hygiene, Kinderspitalgasse 15, A-1090 Vienna, Austriaid="aff8">^h TU Wien, Institute of Chemical Engineering, Research Group Environmental Microbiology and Molecular Ecology, Gumpendorferstraße 1a, A-1060 Vienna, Austria

Recommended Citation:

Philipp Stadler^a^,^b^,^{,et al. Real-time monitoring of beta-d-glucuronidase activity in sediment laden streams: A comparison of prototypes[J]. water Research,2016-01-01,Volume 101}

Service
	Recommend this item
	Sava as my favorate item
	Show this item's statistics
	Export Endnote File
Google Scholar
	Similar articles in Google Scholar
	[Philipp Stadler^a^{]'s Articles}
	[^b^{]'s Articles}
	[^{]'s Articles}
百度学术
	Similar articles in Baidu Scholar
	[Philipp Stadler^a^{]'s Articles}
	[^b^{]'s Articles}
	[^{]'s Articles}
CSDL cross search
	Similar articles in CSDL Cross Search
	[Philipp Stadler^a^{]‘s Articles}
	[^b^{]‘s Articles}
	[^{]‘s Articles}
Related Copyright Policies
Null
收藏/分享