BROMODOMAIN PROTEIN
; MITOSIS
; CHROMATIN
; REACTIVATION
; BINDING
; MARKS
; PLURIPOTENCY
; RECOGNITION
; CHROMOSOMES
; INHIBITION
WOS学科分类:
Cell Biology
WOS研究方向:
Cell Biology
英文摘要:
Global changes in chromatin organization and the cessation of transcription during mitosis are thought to challenge the resumption of appropriate transcription patterns after mitosis. The acetyl-lysine binding protein BRD4 has been previously suggested to function as a transcriptional "bookmark" on mitotic chromatin. Here, genome-wide location analysis of BRD4 in erythroid cells, combined with data normalization and peak characterization approaches, reveals that BRD4 widely occupies mitotic chromatin. However, removal of BRD4 from mitotic chromatin does not impair post-mitotic activation of transcription. Additionally, histone mass spectrometry reveals global preservation of most posttranslational modifications (PTMs) during mitosis. In particular, H3K14ac, H3K27ac, H3K122ac, and H4K16ac widely mark mitotic chromatin, especially at lineage-specific genes, and predict BRD4 mitotic binding genome wide. Therefore, BRD4 is likely not a mitotic bookmark but only a "passenger." Instead, mitotic histone acetylation patterns may constitute the actual bookmarks that restore lineage-specific transcription patterns after mitosis.
1.Childrens Hosp Philadelphia, Div Hematol, Philadelphia, PA 19104 USA 2.Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA 3.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA 4.Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Recommended Citation:
Behera, Vivek,Stonestrom, Aaron J.,Hamagami, Nicole,et al. Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription[J]. CELL REPORTS,2019-01-01,27(2):400-+